Phosphoethanolamine Enhances High‐Affinity Choline Uptake and Acetylcholine Synthesis in Dissociated Cell Cultures of the Rat Septal Nucleus

J. Robert Bostwick, Russell Abbe, Stanley H. Appel

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Dissociated rat septal nucleus cells cultured in defined medium exhibited twofold increases in the maximal rates of sodium-dependent, high-affinity choline uptake and acetylcholine formation when grown in the presence of phosphoethanolamine. The effect was concentration-dependent (EC50 = 15 μM) and appeared to be associated with in vitro maturation of cholinergic neurons rather than with enhanced survival. Choline acetyltransferase, acetylcholinesterase, and choline kinase activities were unaffected by this treatment. The effect of phosphoethanolamine was specific for cholinergic neurons, because treatment with this compound did not alter the kinetic constants for high-affinity neuronal uptake of γ-aminobutyric acid or dopamine. The action appeared to be mediated primarily through activation of the sodium-dependent, high-affinity transport mechanism for choline as opposed to alterations in the storage and release of acetylcholine.

Original languageEnglish (US)
Pages (from-to)236-244
Number of pages9
JournalJournal of Neurochemistry
Volume59
Issue number1
DOIs
StatePublished - Jul 1992

Keywords

  • Acetylcholine synthesis
  • Choline uptake
  • Phosphoethanolamine
  • Tissue culture

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Phosphoethanolamine Enhances High‐Affinity Choline Uptake and Acetylcholine Synthesis in Dissociated Cell Cultures of the Rat Septal Nucleus'. Together they form a unique fingerprint.

Cite this