Phenotypic characterization and viral DNA burden of HIV-infected patients' mononuclear leukocytes that migrate across vascular endothelial barriers in vitro

H. H. Birdgaji, J. Trial, H. J. De Lin A L Jong, D. M. Green, G. Jjorrentino, R. D. Rossen

Research output: Contribution to journalArticlepeer-review

Abstract

We found significantly greater quantities of GDI Ib, CDllc, VLA-4 (CD49d) and activated CDlla (NKI-L16 epitope) on lymphocytes of 39 HIV-infected patients compared with 23 healthy controls. To evaluate whether these alterations are likely to affect transendothelial migration, we measured the numbers and types of leukocytes that move spontaneously across confluent monolayers of endothelium from bovine aorta (BAECs), human umbilical vein (HUVECs), and human brain micro-vessels in the absence of chemotactic stimuli. A significantly greater fraction of the patients' CD4+ T cells migrated across each of these endothelial monolayers during a 2 hr period. Migration correlated with expression of VLA-4. The fraction of CD8-(- T cells or CD144- monocytes that migrated through the same monolayers was not different in patients and controls. Using quantitative PCR, we compared the frequency of proviral HIV DNA in the migratory and original MNL populations. In 7 of 8 patients, the migratory population contained more HIV DNA per million lymphocytes than was found in the starting population. In these 7, the migratory cells contained 10.5 3.3 (SEM)-fold more virus. These results suggest that stimuli, active in vivo, promote alterations in beta-integrin expression, increased migration of CD4+ T cells across endothelial barriers, and the preferential transendothe-liai migration of lymphocytes that contain proviral DNA.

Original languageEnglish (US)
Pages (from-to)A1201
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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