Phage Display Preparation of Specific Polypeptides in Atherosclerotic Foam Cells

Xiang Ji, Dan Liu, Feng Wu, Yu Cen, Lan Ma

Research output: Contribution to journalArticlepeer-review


Atherosclerosis and related complications are the most common causes of death in mod-ern societies. Macrophage‐derived foam cells play critical roles in the initiation and progression of atherosclerosis. Effective, rapid, and instrument‐independent detection in the early stage of chronic atherosclerosis progression could provide an opportunity for early intervention and treatment. Therefore, as a starting point, in this study, we aimed to isolate and prepare foam cell‐specific pol-ypeptides using a phage display platform. The six target polypeptides, which were acquired in this study, were evaluated by ELISA and showed strong specificity with foam cells. Streptavidin coupled quantum dots (QDs) were used as fluorescence developing agents, and images of biotin‐mod-ified polypeptides specifically binding with foam cells were clearly observed. The polypeptides ob-tained in this study could lay the foundation for developing a rapid detection kit for early atherosclerosis lesions and could provide new materials for research on the mechanisms of foam cell formation and the development of blocking drugs.

Original languageEnglish (US)
Article number562
JournalApplied Sciences (Switzerland)
Issue number2
StatePublished - Jan 1 2022


  • Atherosclerosis
  • Foam cell
  • Phage display
  • Specific polypeptide

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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