PET imaging with [11C]PBR28 can localize and quantify upregulated peripheral benzodiazepine receptors associated with cerebral ischemia in rat

Masao Imaizumi, Hyun Ju Kim, Sami S. Zoghbi, Emmanuelle Briard, Jinsoo Hong, John L. Musachio, Christl Ruetzler, De Maw Chuang, Victor W. Pike, Robert B. Innis, Masahiro Fujita

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

Peripheral benzodiazepine receptors (PBRs) are upregulated on activated microglia. We recently developed a promising positron emission tomography (PET) ligand, [11C]PBR28, with high affinity and excellent ratio of specific to nonspecific binding. We assessed the ability of [11C]PBR28 PET to localize PBRs in a rat permanent middle cerebral artery occlusion (MCAO) model of neuroinflammation. [11C]PBR28 was intravenously administered to rats at 4 and 7 days after permanent MCAO. In all experiments, arterial blood was sampled for compartmental modeling of regional distribution volumes, and rat brains were sampled after imaging for in vitro [3H]PK 11195 autoradiography and histological evaluation. [11C]PBR28 PET and [3H]PK 11195 autoradiography showed similar areas of increased PBRs, especially in the peri-ischemic core. Results from these in vivo and in vitro methods were strongly correlated. In this first study to demonstrate neuroinflammation in vivo with small animal PET, [11C]PBR28 had adequate sensitivity to localize and quantify the associated increase in PBRs.

Original languageEnglish (US)
Pages (from-to)200-205
Number of pages6
JournalNeuroscience Letters
Volume411
Issue number3
DOIs
StatePublished - Jan 16 2007

Keywords

  • Activated microglia
  • Cerebral ischemia
  • Neuroinflammation
  • Peripheral benzodiazepine receptor
  • Small animal PET

ASJC Scopus subject areas

  • Neuroscience(all)

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