Peroxidase-like activity of apoferritin paired gold clusters for glucose detection

Xin Jiang, Cuiji Sun, Yi Guo, Guangjun Nie, Li Xu

Research output: Contribution to journalArticle

125 Scopus citations

Abstract

The discovery and application of noble metal nanoclusters have received considerable attention. In this paper, we reported that apoferritin paired gold clusters (Au-Ft) could efficiently catalyze oxidation of 3.3',5.5'-tetramethylbenzidine (TMB) by H2O2 to produce a blue color reaction. Compared with natural enzyme, Au-Ft exhibited higher activity near acidic pH and could be used over a wide range of temperatures. Apoferritin nanocage enhanced the reaction activity of substrate TMB by H2O2. The reaction catalyzed by Au-Ft was found to follow a typical Michaelis-Menten kinetics. The kinetic parameters exhibited a lower Km value (0.097mM) and a higher Kcat value (5.8×104s-1) for TMB than that of horse radish peroxidase (HRP). Base on these findings, Au-Ft, acting as a peroxidase mimetic, performed enzymatic spectrophotometric analysis of glucose. This system exhibited acceptable reproducibility and high selectivity in biosening, suggesting that it could have promising applications in the future.

Original languageEnglish (US)
Pages (from-to)165-170
Number of pages6
JournalBiosensors and Bioelectronics
Volume64
DOIs
StatePublished - Feb 15 2015

Keywords

  • Apoferritin
  • Glucose
  • Gold clusters
  • Kinetic study
  • Peroxidase-like activity

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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