TY - JOUR
T1 - Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy
AU - Daniels, Charlisa R.
AU - Reznik, Carmen
AU - Kilmer, Rachel
AU - Felipe, Mary Jane
AU - Tria, Maria Celeste R
AU - Kourentzi, Katerina
AU - Chen, Wen Hsiang
AU - Advincula, Rigoberto C.
AU - Willson, Richard C.
AU - Landes, Christy F.
N1 - Funding Information:
The authors would like to thank Roderick Pernites of the Advicula group at University of Houston for his initial characterization work. The Welch Grant E-1264, as awarded to Richard Willson, supported the protein work. C. Landes thanks the Norman Hackerman Welch Young Investigator Program at Rice University. The NSF CBET-0854979 and Robert A Welch Foundation (E-1551) as awarded to Rigoberto C. Advincula, supported the PEG linear-dendron and bottle brush synthesis and studies. Acknowledgment is also made to the Donors of the American Chemical Society Petroleum Research Fund for partial support of this research.
PY - 2011/11/1
Y1 - 2011/11/1
N2 - The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.
AB - The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe-PEG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe-PEG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.
KW - Dynamics
KW - Fluorescence correlation spectroscopy (FCS)
KW - Poly(ethylene glycol) (PEG)
KW - Surface interactions
KW - Surface modification
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U2 - 10.1016/j.colsurfb.2011.05.044
DO - 10.1016/j.colsurfb.2011.05.044
M3 - Article
C2 - 21742471
AN - SCOPUS:80052099718
SN - 0927-7765
VL - 88
SP - 31
EP - 38
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -