Peptidoglycan isolated from nontypeable haemophilus influenzae induces experimental otitis media in the chinchilla

Edward R. Leake, Kathy Holmes, David J. Lim, Thomas F. De Maria

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Bacterial cell wall components induce a number of biologic effects and promote inflammatory changes in a variety of hosts. Peptidoglycan isolated from Streptococcus pneumoniae can induce inflammation in the middle ear; however, an analogous role for peptidoglycan derived from gram-negative otitis media pathogens has not been described. Peptidoglycan isolated from nontypeable Haemophilus influenzae (NTHI), a major cause of otitis media, was evaluated in a chinchilla model. The direct injection of the middle ear with 3-300 μg of peptidoglycan resulted in tympanic membrane inflammation, abnormal pressure in the middle ear, leukocytosis, and histopathologic changes in the middle ear mucosa that included marked edema, osteoneogenesis, focal hemorrhage, and a mononuclear infiltration into the subepithelial space. These data indicate that NTHi peptidoglycan induced inflammation and histopathologic changes in the tympanic membrane and middle ear mucosal epithelium and may contribute to the pathogenesis of otitis media.

Original languageEnglish (US)
Pages (from-to)1532-1538
Number of pages7
JournalJournal of Infectious Diseases
Volume170
Issue number6
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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