Penetrating Macrophage-Based Nanoformulation for Periodontitis Treatment

Na Yan, Junchao Xu, Guolin Liu, Chao Ma, Lin Bao, Yalin Cong, Ziyao Wang, Yuliang Zhao, Weihua Xu, Chunying Chen

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Periodontitis is a chronic inflammatory disease caused by the interaction of oral microorganisms with the host immune response. Porphyromonas gingivalis (P.g.) acts as a key mediator in subverting the homeostasis of the local immune system. On the one hand, P.g. inhibits phagocytosis and the killing capacity of immune cells. On the other hand, P.g. increases selective cytokine release, which is beneficial to its further proliferation. Here, we prepared a penetrating macrophage-based nanoformulation (MZ@PNM)-encapsulating hydrogel (MZ@PNM@GCP) that responded to the periodontitis microenvironment. MZ@PNM targeted P.g. via the Toll-like receptor complex 2/1 (TLR2/1) on its macrophage-mimicking membrane, then directly killed P.g. through disruption of bacterial structural integrity by the cationic nanoparticles and intracellular release of an antibacterial drug, metronidazole (MZ). Meanwhile, MZ@PNM interrupted the specific binding of P.g. to immune cells and neutralized complement component 5a (C5a), preventing P.g. subversion of periodontal host immune response. Overall, MZ@PNM@GCP showed potent efficacy in periodontitis treatment, restoring local immune function and killing pathogenic bacteria, while exhibiting favorable biocompatibility, all of which have been demonstrated both in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)18253-18265
Number of pages13
JournalACS Nano
Volume16
Issue number11
DOIs
StatePublished - Nov 22 2022

Keywords

  • Porphyromonas gingivalis
  • host immune dysfunction
  • membrane cloaking nanoparticle
  • periodontitis treatment
  • responsive hydrogel

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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