Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages

M. V. Marshall; T. L. McLemore; R. R. Martin; W. T. Jenkins; D. R. Snodgrass; M. A. Corson; M. S. Arnott; Nelda Wray; A. C. Griffin

doi:10.1016/0304-3835(79)90002-8

Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages

M. V. Marshall, T. L. McLemore, R. R. Martin, W. T. Jenkins, D. R. Snodgrass, M. A. Corson, M. S. Arnott, Nelda Wray, A. C. Griffin

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Abstract

Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [³H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.

Original language	English (US)
Pages (from-to)	103-109
Number of pages	7
Journal	Cancer Letters
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/0304-3835(79)90002-8
State	Published - Dec 1979

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/0304-3835(79)90002-8

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@article{1d208e7625c7418aa3bee48ff88ee98c,

title = "Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages",

abstract = "Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.",

author = "Marshall, {M. V.} and McLemore, {T. L.} and Martin, {R. R.} and Jenkins, {W. T.} and Snodgrass, {D. R.} and Corson, {M. A.} and Arnott, {M. S.} and Nelda Wray and Griffin, {A. C.}",

note = "Funding Information: The assistance of Deborah Harris, Dorothy Haynes and Vicky De Jesus is greatly appreciated. This work was supported, in part, by grants from The Robert A. Welch Foundation (G-035), Biomedical Research Support Grant (RR5511), a grant from the Veteran{\textquoteright}s Administration Hospital, Houston, Texas, and Council for Tobacco Research grants 1102 and 1094. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1979",

month = dec,

doi = "10.1016/0304-3835(79)90002-8",

language = "English (US)",

volume = "8",

pages = "103--109",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages

AU - Marshall, M. V.

AU - McLemore, T. L.

AU - Martin, R. R.

AU - Jenkins, W. T.

AU - Snodgrass, D. R.

AU - Corson, M. A.

AU - Arnott, M. S.

AU - Wray, Nelda

AU - Griffin, A. C.

N1 - Funding Information: The assistance of Deborah Harris, Dorothy Haynes and Vicky De Jesus is greatly appreciated. This work was supported, in part, by grants from The Robert A. Welch Foundation (G-035), Biomedical Research Support Grant (RR5511), a grant from the Veteran’s Administration Hospital, Houston, Texas, and Council for Tobacco Research grants 1102 and 1094. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1979/12

Y1 - 1979/12

N2 - Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.

AB - Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.

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AN - SCOPUS:0018654227

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SP - 103

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages

Abstract

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