Abstract
Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.
Original language | English (US) |
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Pages (from-to) | 103-109 |
Number of pages | 7 |
Journal | Cancer Letters |
Volume | 8 |
Issue number | 2 |
DOIs | |
State | Published - Dec 1979 |
ASJC Scopus subject areas
- Oncology
- Cancer Research