Patterns of benzo[a]pyrene metabolism in normal human pulmonary alveolar macrophages

M. V. Marshall, T. L. McLemore, R. R. Martin, W. T. Jenkins, D. R. Snodgrass, M. A. Corson, M. S. Arnott, Nelda Wray, A. C. Griffin

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Pulmonary alveolar macrophages (PAMs) obtained by bronchopulmonary lavage from 6 normal non-smoking volunteers were incubated with [3H]-benzo[a]pyrene to ascertain the normal metabolism and conjugation of polycyclic aromatic hydrocarbons. Through the use of a crude glucuronidase preparation, both glucuronic acid and sulfate conjugates were examined. Phenols and quinones were identified by high-pressure liquid chromatography as the principal free metabolites formed during 1 h incubation with benzanthracene induced PAMs. In addition, phenols and quinones were major substrates utilized by these cells for conjugation during the incubation period. The ranges of benzo[a]pyrene metabolites produced by PAMs from non-smokers were compiled and the variation in production as well as detoxification of proximate carcinogenic benzo[a]pyrene metabolites are presented.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalCancer Letters
Volume8
Issue number2
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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