Patterning of antibodies using flexographic printing

Christopher O. Phillips, Sridhar Govindarajan, Simon M. Hamblyn, R. Steven Conlan, David T. Gethin, Tim C. Claypole

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Antibodies were patterned onto flexible plastic films using the flexographic printing process. An ink formulation was developed using high molecular weight polyvinyl alcohol in carbonate-bicarbonate buffer. In order to aid both antibody adhesion and the quality of definition in the printed features, a nitrocellulose coating was developed that was capable of being discretely patterned, thus increasing the signal-to-noise ratio of an antibody array. Printing antibody features such as dots, squares, text, and fine lines were reproduced effectively. Furthermore, this process could be easily adapted for printing of other biological materials, including, but not limited to, enzymes, DNA, proteins, aptamers, and cells.

Original languageEnglish (US)
Pages (from-to)9878-9884
Number of pages7
Issue number25
StatePublished - Jun 26 2012

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'Patterning of antibodies using flexographic printing'. Together they form a unique fingerprint.

Cite this