TY - JOUR
T1 - Passive microfluidic devices for cell separation
AU - Zhang, Tianlong
AU - Di Carlo, Dino
AU - Lim, Chwee Teck
AU - Zhou, Tianyuan
AU - Tian, Guizhong
AU - Tang, Tao
AU - Shen, Amy Q.
AU - Li, Weihua
AU - Li, Ming
AU - Yang, Yang
AU - Goda, Keisuke
AU - Yan, Ruopeng
AU - Lei, Cheng
AU - Hosokawa, Yoichiroh
AU - Yalikun, Yaxiaer
N1 - Publisher Copyright:
© 2024
PY - 2024/3/1
Y1 - 2024/3/1
N2 - The separation of specific cell populations is instrumental in gaining insights into cellular processes, elucidating disease mechanisms, and advancing applications in tissue engineering, regenerative medicine, diagnostics, and cell therapies. Microfluidic methods for cell separation have propelled the field forward, benefitting from miniaturization, advanced fabrication technologies, a profound understanding of fluid dynamics governing particle separation mechanisms, and a surge in interdisciplinary investigations focused on diverse applications. Cell separation methodologies can be categorized according to their underlying separation mechanisms. Passive microfluidic separation systems rely on channel structures and fluidic rheology, obviating the necessity for external force fields to facilitate label-free cell separation. These passive approaches offer a compelling combination of cost-effectiveness and scalability when compared to active methods that depend on external fields to manipulate cells. This review delves into the extensive utilization of passive microfluidic techniques for cell separation, encompassing various strategies such as filtration, sedimentation, adhesion-based techniques, pinched flow fractionation (PFF), deterministic lateral displacement (DLD), inertial microfluidics, hydrophoresis, viscoelastic microfluidics, and hybrid microfluidics. Besides, the review provides an in-depth discussion concerning cell types, separation markers, and the commercialization of these technologies. Subsequently, it outlines the current challenges faced in the field and presents a forward-looking perspective on potential future developments. This work hopes to aid in facilitating the dissemination of knowledge in cell separation, guiding future research, and informing practical applications across diverse scientific disciplines.
AB - The separation of specific cell populations is instrumental in gaining insights into cellular processes, elucidating disease mechanisms, and advancing applications in tissue engineering, regenerative medicine, diagnostics, and cell therapies. Microfluidic methods for cell separation have propelled the field forward, benefitting from miniaturization, advanced fabrication technologies, a profound understanding of fluid dynamics governing particle separation mechanisms, and a surge in interdisciplinary investigations focused on diverse applications. Cell separation methodologies can be categorized according to their underlying separation mechanisms. Passive microfluidic separation systems rely on channel structures and fluidic rheology, obviating the necessity for external force fields to facilitate label-free cell separation. These passive approaches offer a compelling combination of cost-effectiveness and scalability when compared to active methods that depend on external fields to manipulate cells. This review delves into the extensive utilization of passive microfluidic techniques for cell separation, encompassing various strategies such as filtration, sedimentation, adhesion-based techniques, pinched flow fractionation (PFF), deterministic lateral displacement (DLD), inertial microfluidics, hydrophoresis, viscoelastic microfluidics, and hybrid microfluidics. Besides, the review provides an in-depth discussion concerning cell types, separation markers, and the commercialization of these technologies. Subsequently, it outlines the current challenges faced in the field and presents a forward-looking perspective on potential future developments. This work hopes to aid in facilitating the dissemination of knowledge in cell separation, guiding future research, and informing practical applications across diverse scientific disciplines.
KW - Cell separation
KW - Hybrid
KW - Intelligent
KW - Label-free
KW - Passive microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85182872687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85182872687&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2024.108317
DO - 10.1016/j.biotechadv.2024.108317
M3 - Review article
C2 - 38220118
AN - SCOPUS:85182872687
SN - 0734-9750
VL - 71
JO - Biotechnology Advances
JF - Biotechnology Advances
M1 - 108317
ER -