Particle velocity measurements with macroscopic fluorescence imaging in lymph tissue mimicking microfluidic phantoms

Ricky Hennessy, Chiwan Koo, Phuc Ton, Arum Han, Raffaella Righetti, Kristen C. Maitland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


Ultrasound poroelastography can quantify structural and mechanical properties of tissues such as stiffness, compressibility, and fluid flow rate. This novel ultrasound technique is being explored to detect tissue changes associated with lymphatic disease. We have constructed a macroscopic fluorescence imaging system to validate ultrasonic fluid flow measurements and to provide high resolution imaging of microfluidic phantoms. The optical imaging system is composed of a white light source, excitation and emission filters, and a camera with a zoom lens. The field of view can be adjusted from 100 mm x 75 mm to 10 mm x 7.5 mm. The microfluidic device is made of polydimethylsiloxane (PDMS) and has 9 channels, each 40 μm deep with widths ranging from 30 μm to 200 μm. A syringe pump was used to propel water containing 15 μm diameter fluorescent microspheres through the microchannels, with flow rates ranging from 0.5 μl/min to 10 μl/min. Video was captured at a rate of 25 frames/sec. The velocity of the microspheres in the microchannels was calculated using an algorithm that tracked the movement of the fluorescent microspheres. The imaging system was able to measure particle velocities ranging from 0.2 mm/sec to 10 mm/sec. The range of flow velocities of interest in lymph vessels is between 1 mm/sec to 10 mm/sec; therefore our imaging system is sufficient to measure particle velocity in phantoms modeling lymphatic flow.

Original languageEnglish (US)
Title of host publicationMultimodal Biomedical Imaging VI
StatePublished - May 2 2011
EventMultimodal Biomedical Imaging VI - San Francisco, CA, United States
Duration: Jan 22 2011Jan 23 2011


OtherMultimodal Biomedical Imaging VI
Country/TerritoryUnited States
CitySan Francisco, CA


  • fluid flow
  • fluorescence imaging
  • lymph vessels
  • lymphedema
  • microfluidic
  • ultrasound poroelastography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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