TY - JOUR
T1 - P53-inducible long non-coding RNA PICART1 mediates cancer cell proliferation and migration
AU - Cao, Yu
AU - Lin, Minglin
AU - Bu, Yiwen
AU - Hongyan, Ling
AU - He, Yingchun
AU - Chenfei, Huang
AU - Shen, Yi
AU - Song, Bob
AU - Cao, Deliang
N1 - Funding Information:
This research was supported in part by the National Natural Science Foundation of China (81272918 and 81472465 to D.C.).
PY - 2017/5
Y1 - 2017/5
N2 - Long non-coding RNAs (lncRNAs) function in the development and progression of cancer, but only a small portion of lncRNAs have been characterized to date. A novel lncRNA transcript, 2.53 kb in length, was identified by transcriptome sequencing analysis, and was named p53-inducible cancer-associated RNA transcript 1 (PICART1). PICART1 was found to be upregulated by p53 through a p53-binding site at -1808 to -1783 bp. In breast and colorectal cancer cells and tissues, PICART1 expression was found to be decreased. Ectopic expression of PICART1 suppressed the growth, proliferation, migration, and invasion of MCF7, MDA-MB-231 and HCT116 cells whereas silencing of PICART1 stimulated cell growth and migration. In these cells, the expression of PICART1 suppressed levels of p-AKT (Thr308 and Ser473) and p-GSK3β (Ser9), and accordingly, β-catenin, cyclin D1 and c-Myc expression were decreased, while p21Waf/cip1 expression was increased. Together these data suggest that PICART1 is a novel p53-inducible tumor-suppressor lncRNA, functioning through the AKT/GSK3β/β-catenin signaling cascade.
AB - Long non-coding RNAs (lncRNAs) function in the development and progression of cancer, but only a small portion of lncRNAs have been characterized to date. A novel lncRNA transcript, 2.53 kb in length, was identified by transcriptome sequencing analysis, and was named p53-inducible cancer-associated RNA transcript 1 (PICART1). PICART1 was found to be upregulated by p53 through a p53-binding site at -1808 to -1783 bp. In breast and colorectal cancer cells and tissues, PICART1 expression was found to be decreased. Ectopic expression of PICART1 suppressed the growth, proliferation, migration, and invasion of MCF7, MDA-MB-231 and HCT116 cells whereas silencing of PICART1 stimulated cell growth and migration. In these cells, the expression of PICART1 suppressed levels of p-AKT (Thr308 and Ser473) and p-GSK3β (Ser9), and accordingly, β-catenin, cyclin D1 and c-Myc expression were decreased, while p21Waf/cip1 expression was increased. Together these data suggest that PICART1 is a novel p53-inducible tumor-suppressor lncRNA, functioning through the AKT/GSK3β/β-catenin signaling cascade.
KW - AKT
KW - GSK3β
KW - Long non-coding RNA
KW - P53
KW - PICART1
KW - Tumor suppressor
KW - β-catenin
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U2 - 10.3892/ijo.2017.3918
DO - 10.3892/ijo.2017.3918
M3 - Article
C2 - 28339031
AN - SCOPUS:85018466787
SN - 1019-6439
VL - 50
SP - 1671
EP - 1682
JO - International Journal of Oncology
JF - International Journal of Oncology
IS - 5
ER -