p53 Gene Mutations in Barrett's Epithelium and Esophageal Cancer

Alan G. Casson, Tapas Mukhopadhyay, Karen R. Cleary, Jae Y. Ro, Bernard Levin, Jack A. Roth

Research output: Contribution to journalArticle

239 Scopus citations

Abstract

Genomic DNA was extracted from archival pathology specimens comprising 10 squamous and 14 adenocarcinomas, including 7 with Barrett's epithelium adjacent to tumor, and corresponding normal esophagus from the resection margin. The polymerase chain reaction was used to amplify selected exons of p53 which were analyzed for mutations using single-strand conformation polymorphism analysis. Mutations were localized to exon 8 for 1 adenocarcinoma and to exon 5 for 1 squamous tumor and 4 of 7 Barrett's specimens. Sequencing confirmed mutations at codons 273 (CGT→CAT; adenocarcinoma) and 176 (TGC→TTC; squamous) and in Barrett's epithelium at codons 152 (CCG→CTG), 155 (ACC→GCC) and 175 (CGC→CAC). Specimens of Barrett's epithelium from separate sites had identical p53 mutations suggesting a clonal origin. Cancers arising in mutant epithelium did not have mutations corresponding to those found in the Barrett's specimens suggesting that other events are required for tumorigenesis.

Original languageEnglish (US)
Pages (from-to)4495-4499
Number of pages5
JournalCancer research
Volume51
Issue number16
StatePublished - Aug 15 1991

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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