Overexpression of small glutamine-rich TPR-containing protein promotes apoptosis in 7721 cells

Hanzhou Wang, Hailian Shen, Yanlin Wang, Zejuan Li, Hongyan Yin, Hongliang Zong, Jianhai Jiang, Jianxin Gu

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

It is known that small glutamine-rich TPR-containing protein (SGT) is the member of TPR motif family. However, the biological functions of SGT remain unclear. In this paper, we report that SGT plays a role in apoptotic signaling. Ectopic expression of SGT enhances DNA fragment and nucleus breakage after the induction of apoptosis. Increasing mRNA level of SGT is also observed in 7721 cells undergoing apoptosis, knockdown the expression of endogenous SGT contributes to the decrease of apoptosis of 7721 cells. Deletion analysis reveals that TPR domain is critical to pro-apoptotic function of SGT. Furthermore, we demonstrated that the PARP cleavage and cytochrome c release are enhanced when SGT is overexpressed in 7721 cells during apoptosis. Collectively, our results indicate that SGT is a new pro-apoptotic factor.

Original languageEnglish (US)
Pages (from-to)1279-1284
Number of pages6
JournalFEBS Letters
Volume579
Issue number5
DOIs
StatePublished - Feb 14 2005

Keywords

  • Apoptosis
  • Heat shock cognate protein 70
  • Heat shock protein 70
  • Small glutamine-rich TPR-containing protein
  • TPR

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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