Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures

Changgeng Peng; Songhua Fan; Xuping Li; Xiaolan Fan; Ming Ming; Zhe Sun; Weidong Le

doi:10.1016/j.febslet.2007.02.054

Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures

Changgeng Peng, Songhua Fan, Xuping Li, Xiaolan Fan, Ming Ming, Zhe Sun, Weidong Le

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The transcription factor Pitx3 plays an important role in the development of midbrain to promote the growth and differentiation of dopamine neurons. The present study has demonstrated that overexpression of Pitx3 in SH-SY5Y cells and primary ventral mesencephalic (VM) cultures significantly increased the mRNA levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), and remarkably elevated the protein levels of these two neurotrophic factors. Our data provide the first evidence that pitx3-expressing cells are able to upregulate the expression of BDNF and GDNF. Therefore, Pitx3 might be a good target for the treatment of Parkinson's disease.

Original language	English (US)
Pages (from-to)	1357-1361
Number of pages	5
Journal	FEBS Letters
Volume	581
Issue number	7
DOIs	https://doi.org/10.1016/j.febslet.2007.02.054
State	Published - Apr 3 2007

Keywords

Brain-derived neurotrophic factor
Glial cell line-derived neurotrophic factor
Pitx3
SH-SY5Y cells
Ventral mesencephalon cultures

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2007.02.054

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title = "Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures",

abstract = "The transcription factor Pitx3 plays an important role in the development of midbrain to promote the growth and differentiation of dopamine neurons. The present study has demonstrated that overexpression of Pitx3 in SH-SY5Y cells and primary ventral mesencephalic (VM) cultures significantly increased the mRNA levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), and remarkably elevated the protein levels of these two neurotrophic factors. Our data provide the first evidence that pitx3-expressing cells are able to upregulate the expression of BDNF and GDNF. Therefore, Pitx3 might be a good target for the treatment of Parkinson's disease.",

keywords = "Brain-derived neurotrophic factor, Glial cell line-derived neurotrophic factor, Pitx3, SH-SY5Y cells, Ventral mesencephalon cultures",

author = "Changgeng Peng and Songhua Fan and Xuping Li and Xiaolan Fan and Ming Ming and Zhe Sun and Weidong Le",

note = "Funding Information: This work was supported by a Research Fund (05DJ14007) from Science and Technology Commission of Shanghai Municipality, and a Research Fund (E03003) from High Education Commission of Shanghai Municipality.",

year = "2007",

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doi = "10.1016/j.febslet.2007.02.054",

language = "English (US)",

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T1 - Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures

AU - Peng, Changgeng

AU - Fan, Songhua

AU - Li, Xuping

AU - Fan, Xiaolan

AU - Ming, Ming

AU - Sun, Zhe

AU - Le, Weidong

N1 - Funding Information: This work was supported by a Research Fund (05DJ14007) from Science and Technology Commission of Shanghai Municipality, and a Research Fund (E03003) from High Education Commission of Shanghai Municipality.

PY - 2007/4/3

Y1 - 2007/4/3

N2 - The transcription factor Pitx3 plays an important role in the development of midbrain to promote the growth and differentiation of dopamine neurons. The present study has demonstrated that overexpression of Pitx3 in SH-SY5Y cells and primary ventral mesencephalic (VM) cultures significantly increased the mRNA levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), and remarkably elevated the protein levels of these two neurotrophic factors. Our data provide the first evidence that pitx3-expressing cells are able to upregulate the expression of BDNF and GDNF. Therefore, Pitx3 might be a good target for the treatment of Parkinson's disease.

AB - The transcription factor Pitx3 plays an important role in the development of midbrain to promote the growth and differentiation of dopamine neurons. The present study has demonstrated that overexpression of Pitx3 in SH-SY5Y cells and primary ventral mesencephalic (VM) cultures significantly increased the mRNA levels of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF), and remarkably elevated the protein levels of these two neurotrophic factors. Our data provide the first evidence that pitx3-expressing cells are able to upregulate the expression of BDNF and GDNF. Therefore, Pitx3 might be a good target for the treatment of Parkinson's disease.

KW - Brain-derived neurotrophic factor

KW - Glial cell line-derived neurotrophic factor

KW - Pitx3

KW - SH-SY5Y cells

KW - Ventral mesencephalon cultures

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

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Overexpression of pitx3 upregulates expression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephalic cultures

Abstract

Keywords

ASJC Scopus subject areas

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