Overexpression of genes in the CA1 hippocampus region of adult rat following episodes of global ischemia

E. Yakubov, M. Gottlieb, S. Gil, P. Dinerman, P. Fuchs, E. Yavin

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Ischemic stress is associated with marked changes in gene expression in the hippocampus - albeit little information exists on the activation of nonabundant genes. We have examined the expression of several known genes and identified novel ones in the adult rat hippocampus after a mild, transient, hypovolemic and hypotensive, global ischemic stress. An initial differential screening using a prototype array to assess gene expression after stress followed by a suppression subtractive hybridization protocol and cDNA microarray revealed 124 nonoverlapped transcripts predominantly expressed in the CA1 rat hippocampus region in response to ischemic stress. About 78% of these genes were not detected with nonsubtracted probes. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization on these 124 transcripts confirmed the differential expression of at least 83. Most robustly expressed were gene sequences NFI-B, ATP1B1, RHOGAP, PLA2G4A, BAX, CASP3, P53, MAO-A, FRA1, HSP70.2, and NR4A1 (NUR77), as well as sequence tags of unknown function. New stress-related genes of similar functional motifs were identified, reemphasizing the importance of functional grouping in the analysis of multiple gene expression profiles. These data indicate that ischemia elicits expression of an array of functional gene clusters that may be used as an index for stress severity and a template for target therapy design.

Original languageEnglish (US)
Pages (from-to)10-26
Number of pages17
JournalMolecular Brain Research
Volume127
Issue number1-2
DOIs
StatePublished - Aug 23 2004

Keywords

  • 2VO
  • Cell death
  • Cellular and molecular biology
  • Gene clusters
  • Gene structure and function: general
  • gene-specific primer
  • Global ischemia
  • GSP
  • hVhT
  • hypovolemic/hypotensive
  • Microarray
  • RT
  • Stress
  • Suppression subtractive hybridization
  • two-vessel occlusion

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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