TY - JOUR
T1 - Overexpression of genes in the CA1 hippocampus region of adult rat following episodes of global ischemia
AU - Yakubov, E.
AU - Gottlieb, M.
AU - Gil, S.
AU - Dinerman, P.
AU - Fuchs, P.
AU - Yavin, E.
N1 - Funding Information:
This work was supported by a grant from the Gulton Foundation (New York) and by the Nella and Leon Benozyio Center for Neurosciences. E.Y. is incumbent of the Bee Wiggs Professorial Chair in Molecular Biology at the Weizmann Institute. The authors are grateful to Dr. A. Brand for critical reading of the manuscript.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/8/23
Y1 - 2004/8/23
N2 - Ischemic stress is associated with marked changes in gene expression in the hippocampus - albeit little information exists on the activation of nonabundant genes. We have examined the expression of several known genes and identified novel ones in the adult rat hippocampus after a mild, transient, hypovolemic and hypotensive, global ischemic stress. An initial differential screening using a prototype array to assess gene expression after stress followed by a suppression subtractive hybridization protocol and cDNA microarray revealed 124 nonoverlapped transcripts predominantly expressed in the CA1 rat hippocampus region in response to ischemic stress. About 78% of these genes were not detected with nonsubtracted probes. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization on these 124 transcripts confirmed the differential expression of at least 83. Most robustly expressed were gene sequences NFI-B, ATP1B1, RHOGAP, PLA2G4A, BAX, CASP3, P53, MAO-A, FRA1, HSP70.2, and NR4A1 (NUR77), as well as sequence tags of unknown function. New stress-related genes of similar functional motifs were identified, reemphasizing the importance of functional grouping in the analysis of multiple gene expression profiles. These data indicate that ischemia elicits expression of an array of functional gene clusters that may be used as an index for stress severity and a template for target therapy design.
AB - Ischemic stress is associated with marked changes in gene expression in the hippocampus - albeit little information exists on the activation of nonabundant genes. We have examined the expression of several known genes and identified novel ones in the adult rat hippocampus after a mild, transient, hypovolemic and hypotensive, global ischemic stress. An initial differential screening using a prototype array to assess gene expression after stress followed by a suppression subtractive hybridization protocol and cDNA microarray revealed 124 nonoverlapped transcripts predominantly expressed in the CA1 rat hippocampus region in response to ischemic stress. About 78% of these genes were not detected with nonsubtracted probes. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization on these 124 transcripts confirmed the differential expression of at least 83. Most robustly expressed were gene sequences NFI-B, ATP1B1, RHOGAP, PLA2G4A, BAX, CASP3, P53, MAO-A, FRA1, HSP70.2, and NR4A1 (NUR77), as well as sequence tags of unknown function. New stress-related genes of similar functional motifs were identified, reemphasizing the importance of functional grouping in the analysis of multiple gene expression profiles. These data indicate that ischemia elicits expression of an array of functional gene clusters that may be used as an index for stress severity and a template for target therapy design.
KW - 2VO
KW - Cell death
KW - Cellular and molecular biology
KW - Gene clusters
KW - Gene structure and function: general
KW - gene-specific primer
KW - Global ischemia
KW - GSP
KW - hVhT
KW - hypovolemic/hypotensive
KW - Microarray
KW - RT
KW - Stress
KW - Suppression subtractive hybridization
KW - two-vessel occlusion
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U2 - 10.1016/j.molbrainres.2004.05.010
DO - 10.1016/j.molbrainres.2004.05.010
M3 - Article
C2 - 15306117
AN - SCOPUS:4043076946
VL - 127
SP - 10
EP - 26
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0169-328X
IS - 1-2
ER -