O6-Methylguanine-DNA methyltransferase in human cells

Daniel B. Yarosh, Mary Rice, Rufus S. Day, Robert S. Foote, Sankar Mitra

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

O6-Methylguanine-DNA methyltransferase activity was measured in extracts of human tumor cells and was partially purified from human placenta. Repair of O6-methylguanine in DNA inactivated the methyltransferase, and treatment of cells with MNNG, which produces this alkylated base in DNA, depleted the cells of active methyltransferase. RNA and protein synthesis were required for restoration of methyltransferase activity, which transiently exceeded the original levels by 50% 48 h after treatment. One species of methyltransferase of Mr = 22 kd was present in human tumor cells and human placenta.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalMutation Research DNA Repair Reports
Volume131
Issue number1
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

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