Organization of the mouse microfibril-associated glycoprotein-2 (MAGP- 2) gene

Cheryl Frankfater, Erika Maus, Krisztina Gaal, Fernando Segade, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, J. Michael Shipley

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains 10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3' untranslated sequence, including a B2 repetitive element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide repeat sequence located ~155 bp 5' to exon 1.

Original languageEnglish (US)
Pages (from-to)191-195
Number of pages5
JournalMammalian Genome
Volume11
Issue number3
DOIs
StatePublished - Mar 15 2000

ASJC Scopus subject areas

  • Genetics

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