Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity

Research output: Contribution to journalArticle

Annalisa Aluigi, Marco Ballestri, Andrea Guerrini, Giovanna Sotgiu, Claudia Ferroni, Franco Corticelli, Marzia Bruna Gariboldi, Elena Monti, Greta Varchi

Doxorubicin is one of the most effective chemotherapeutic agents for the treatment of several neoplastic conditions, such as leukemia, neuroblastoma, soft tissue and bone sarcomas, breast cancer, ovarian cancer and others. However, its clinical application is limited by cardiotoxicity, such as cardiomyopathy, that once developed carries a poor prognosis and is frequently fatal. The controlled release of doxorubicin by means of a smart carrier is a strategy to overcome the aforementioned drawback. Herein, doxorubicin/keratin nanoparticles were prepared by loading the drug through ionic gelation and aggregation methods, without using cross linkers, organic solvents neither surfactants. Both methodologies afford nanoparticles with yields up to 100 wt%, depending on the loading amount of doxorubicin. Although aggregation yield smaller nanoparticles (≈100 nm), ionic gelation allows a higher drug loading (up to 30 wt%,). More importantly, nanoparticles obtained through this procedure display a pH-responsive release of the drug: indeed Peppas-Salhin model suggests that, the doxorubicin release mechanism is predominantly controlled by diffusion at pH 7.4 and by protein swelling at pH 5. Moreover, nanoparticles prepared by ionic gelation resulted in more efficient cell killing of MDA-MB-231 and MCF-7 breast cancer cells than those prepared by aggregation. Based on the herein presented preliminary results, ionic gelation emerges as a promising approach for the preparation of keratin-based doxorubicin nanocarriers for cancer therapy, that is worth to further investigate.

Original languageEnglish (US)
Pages (from-to)476-484
Number of pages9
JournalMaterials Science and Engineering C
Volume90
DOIs
StatePublished - Sep 1 2018

PMID: 29853116

Altmetrics

Cite this

Standard

Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity. / Aluigi, Annalisa; Ballestri, Marco; Guerrini, Andrea; Sotgiu, Giovanna; Ferroni, Claudia; Corticelli, Franco; Gariboldi, Marzia Bruna; Monti, Elena; Varchi, Greta.

In: Materials Science and Engineering C, Vol. 90, 01.09.2018, p. 476-484.

Research output: Contribution to journalArticle

Harvard

Aluigi, A, Ballestri, M, Guerrini, A, Sotgiu, G, Ferroni, C, Corticelli, F, Gariboldi, MB, Monti, E & Varchi, G 2018, 'Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity' Materials Science and Engineering C, vol. 90, pp. 476-484. DOI: 10.1016/j.msec.2018.04.088

APA

Aluigi, A., Ballestri, M., Guerrini, A., Sotgiu, G., Ferroni, C., Corticelli, F., ... Varchi, G. (2018). Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity. Materials Science and Engineering C, 90, 476-484. DOI: 10.1016/j.msec.2018.04.088

Vancouver

Aluigi A, Ballestri M, Guerrini A, Sotgiu G, Ferroni C, Corticelli F et al. Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity. Materials Science and Engineering C. 2018 Sep 1;90:476-484. Available from, DOI: 10.1016/j.msec.2018.04.088

Author

Aluigi, Annalisa ; Ballestri, Marco ; Guerrini, Andrea ; Sotgiu, Giovanna ; Ferroni, Claudia ; Corticelli, Franco ; Gariboldi, Marzia Bruna ; Monti, Elena ; Varchi, Greta. / Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity. In: Materials Science and Engineering C. 2018 ; Vol. 90. pp. 476-484

BibTeX

@article{3196a6a640764e2f873343cfc86c4652,
title = "Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity",
abstract = "Doxorubicin is one of the most effective chemotherapeutic agents for the treatment of several neoplastic conditions, such as leukemia, neuroblastoma, soft tissue and bone sarcomas, breast cancer, ovarian cancer and others. However, its clinical application is limited by cardiotoxicity, such as cardiomyopathy, that once developed carries a poor prognosis and is frequently fatal. The controlled release of doxorubicin by means of a smart carrier is a strategy to overcome the aforementioned drawback. Herein, doxorubicin/keratin nanoparticles were prepared by loading the drug through ionic gelation and aggregation methods, without using cross linkers, organic solvents neither surfactants. Both methodologies afford nanoparticles with yields up to 100 wt{\%}, depending on the loading amount of doxorubicin. Although aggregation yield smaller nanoparticles (≈100 nm), ionic gelation allows a higher drug loading (up to 30 wt{\%},). More importantly, nanoparticles obtained through this procedure display a pH-responsive release of the drug: indeed Peppas-Salhin model suggests that, the doxorubicin release mechanism is predominantly controlled by diffusion at pH 7.4 and by protein swelling at pH 5. Moreover, nanoparticles prepared by ionic gelation resulted in more efficient cell killing of MDA-MB-231 and MCF-7 breast cancer cells than those prepared by aggregation. Based on the herein presented preliminary results, ionic gelation emerges as a promising approach for the preparation of keratin-based doxorubicin nanocarriers for cancer therapy, that is worth to further investigate.",
keywords = "Aggregation, Doxorubicin, Ionic gelation, Keratin, Nanoparticles",
author = "Annalisa Aluigi and Marco Ballestri and Andrea Guerrini and Giovanna Sotgiu and Claudia Ferroni and Franco Corticelli and Gariboldi, {Marzia Bruna} and Elena Monti and Greta Varchi",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.msec.2018.04.088",
language = "English (US)",
volume = "90",
pages = "476--484",
journal = "Materials Science and Engineering C",
issn = "0928-4931",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Organic solvent-free preparation of keratin nanoparticles as doxorubicin carriers for antitumour activity

AU - Aluigi,Annalisa

AU - Ballestri,Marco

AU - Guerrini,Andrea

AU - Sotgiu,Giovanna

AU - Ferroni,Claudia

AU - Corticelli,Franco

AU - Gariboldi,Marzia Bruna

AU - Monti,Elena

AU - Varchi,Greta

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Doxorubicin is one of the most effective chemotherapeutic agents for the treatment of several neoplastic conditions, such as leukemia, neuroblastoma, soft tissue and bone sarcomas, breast cancer, ovarian cancer and others. However, its clinical application is limited by cardiotoxicity, such as cardiomyopathy, that once developed carries a poor prognosis and is frequently fatal. The controlled release of doxorubicin by means of a smart carrier is a strategy to overcome the aforementioned drawback. Herein, doxorubicin/keratin nanoparticles were prepared by loading the drug through ionic gelation and aggregation methods, without using cross linkers, organic solvents neither surfactants. Both methodologies afford nanoparticles with yields up to 100 wt%, depending on the loading amount of doxorubicin. Although aggregation yield smaller nanoparticles (≈100 nm), ionic gelation allows a higher drug loading (up to 30 wt%,). More importantly, nanoparticles obtained through this procedure display a pH-responsive release of the drug: indeed Peppas-Salhin model suggests that, the doxorubicin release mechanism is predominantly controlled by diffusion at pH 7.4 and by protein swelling at pH 5. Moreover, nanoparticles prepared by ionic gelation resulted in more efficient cell killing of MDA-MB-231 and MCF-7 breast cancer cells than those prepared by aggregation. Based on the herein presented preliminary results, ionic gelation emerges as a promising approach for the preparation of keratin-based doxorubicin nanocarriers for cancer therapy, that is worth to further investigate.

AB - Doxorubicin is one of the most effective chemotherapeutic agents for the treatment of several neoplastic conditions, such as leukemia, neuroblastoma, soft tissue and bone sarcomas, breast cancer, ovarian cancer and others. However, its clinical application is limited by cardiotoxicity, such as cardiomyopathy, that once developed carries a poor prognosis and is frequently fatal. The controlled release of doxorubicin by means of a smart carrier is a strategy to overcome the aforementioned drawback. Herein, doxorubicin/keratin nanoparticles were prepared by loading the drug through ionic gelation and aggregation methods, without using cross linkers, organic solvents neither surfactants. Both methodologies afford nanoparticles with yields up to 100 wt%, depending on the loading amount of doxorubicin. Although aggregation yield smaller nanoparticles (≈100 nm), ionic gelation allows a higher drug loading (up to 30 wt%,). More importantly, nanoparticles obtained through this procedure display a pH-responsive release of the drug: indeed Peppas-Salhin model suggests that, the doxorubicin release mechanism is predominantly controlled by diffusion at pH 7.4 and by protein swelling at pH 5. Moreover, nanoparticles prepared by ionic gelation resulted in more efficient cell killing of MDA-MB-231 and MCF-7 breast cancer cells than those prepared by aggregation. Based on the herein presented preliminary results, ionic gelation emerges as a promising approach for the preparation of keratin-based doxorubicin nanocarriers for cancer therapy, that is worth to further investigate.

KW - Aggregation

KW - Doxorubicin

KW - Ionic gelation

KW - Keratin

KW - Nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85046459393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046459393&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2018.04.088

DO - 10.1016/j.msec.2018.04.088

M3 - Article

VL - 90

SP - 476

EP - 484

JO - Materials Science and Engineering C

T2 - Materials Science and Engineering C

JF - Materials Science and Engineering C

SN - 0928-4931

ER -

ID: 39683166