Ordered arrays of native chromatin molecules for high-resolution imaging and analysis

Aline Cerf, Harvey C. Tian, Harold G. Craighead

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Individual chromatin molecules contain valuable genetic and epigenetic information. To date, there have not been reliable techniques available for the controlled stretching and manipulation of individual chromatin fragments for high-resolution imaging and analysis of these molecules. We report the controlled stretching of single chromatin fragments extracted from two different cancerous cell types (M091 and HeLa) characterized through fluorescence microscopy and atomic force microscopy (AFM). Our method combines soft lithography with molecular stretching to form ordered arrays of more than 250 000 individual chromatin fragments immobilized into a beads-on-a-string structure on a solid transparent support. Using fluorescence microscopy and AFM, we verified the presence of histone proteins after the stretching and transfer process.

Original languageEnglish (US)
Pages (from-to)7928-7934
Number of pages7
JournalACS Nano
Volume6
Issue number9
DOIs
StatePublished - Sep 25 2012

Keywords

  • AFM
  • fluorescence imaging
  • mammalian chromatin
  • single molecule
  • soft lithography .molecular stretching

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

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