OR-1, a member of the nuclear receptor superfamily that interacts with the 9-cis-retinoic acid receptor

M. Teboul, E. Enmark, Q. Li, A. C. Wikström, M. Pelto-Huikko, J. Å Gustafsson

Research output: Contribution to journalArticlepeer-review

193 Scopus citations

Abstract

We have cloned a member of the nuclear receptor superfamily. The cDNA was isolated from a rat liver library and encodes a protein of 446 aa with a predicted mass of 50 kDa. This clone (OR-1) shows no striking homology to any known member of the steroid/thyroid hormone receptor superfamily. The most related receptor is the ecdysone receptor and the highest homologies represent <10% in the amino-terminal domain, between 15-37% in the carboxyl- terminal domain and 50-62% in the DNA binding domain. The expression of OR-1 appears to be widespread in both fetal and adult rat tissues. Potential DNA response elements composed of a direct repeat of the hexameric motif AGGTCA spaced by 0-6 nt were tested in gel shift experiments. OR-1 was shown to interact with the 9-cis-retinoic acid receptor (retinoid X receptor, RXR) and the OR-1/RXR complex to bind to a direct repeat spaced by 4 nt (DR4). In transfection experiments, OR-1 appears to activate RXR-mediated function through the DR4. Therefore OR-1 might modulate 9-cis-retinoic acid signaling by interacting with RXR.

Original languageEnglish (US)
Pages (from-to)2096-2100
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number6
DOIs
StatePublished - Mar 14 1995

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'OR-1, a member of the nuclear receptor superfamily that interacts with the 9-cis-retinoic acid receptor'. Together they form a unique fingerprint.

Cite this