Optogenetic probing of functional brain circuitry

James J. Mancuso; Jinsook Kim; Soojung Lee; Sachiko Tsuda; Nicholas B.H. Chow; George J. Augustine

doi:10.1113/expphysiol.2010.055731

Optogenetic probing of functional brain circuitry

James J. Mancuso, Jinsook Kim, Soojung Lee, Sachiko Tsuda, Nicholas B.H. Chow, George J. Augustine

Academic Institute

Research output: Contribution to journal › Review article › peer-review

54 Scopus citations

Abstract

Recently developed optogenetic technologies offer the promise of high-speed mapping of brain circuitry. Genetically targeted light-gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic probes of neuronal activity, such as Clomeleon and Mermaid, allow light to be used to monitor the activity of a genetically defined population of neurons. Combining these two complementary sets of optogenetic probes will make it possible to perform all-optical circuit mapping. Owing to the improved efficiency and higher speed of data acquisition, this hybrid approach should enable high-throughput mapping of brain circuitry.

Original language	English (US)
Pages (from-to)	26-33
Number of pages	8
Journal	Experimental Physiology
Volume	96
Issue number	1
DOIs	https://doi.org/10.1113/expphysiol.2010.055731
State	Published - Jan 1 2011

ASJC Scopus subject areas

Physiology
Nutrition and Dietetics
Physiology (medical)

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10.1113/expphysiol.2010.055731

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AU - Kim, Jinsook

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AU - Chow, Nicholas B.H.

AU - Augustine, George J.

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AB - Recently developed optogenetic technologies offer the promise of high-speed mapping of brain circuitry. Genetically targeted light-gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic probes of neuronal activity, such as Clomeleon and Mermaid, allow light to be used to monitor the activity of a genetically defined population of neurons. Combining these two complementary sets of optogenetic probes will make it possible to perform all-optical circuit mapping. Owing to the improved efficiency and higher speed of data acquisition, this hybrid approach should enable high-throughput mapping of brain circuitry.

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Optogenetic probing of functional brain circuitry

Abstract

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