TY - JOUR
T1 - Optimized production of a high-performance hybrid biomaterial
T2 - biomineralized spider silk for bone tissue engineering
AU - Dellaquila, Alessandra
AU - Greco, Gabriele
AU - Campodoni, Elisabetta
AU - Mazzocchi, Mauro
AU - Mazzolai, Barbara
AU - Tampieri, Anna
AU - Pugno, Nicola M.
AU - Sandri, Monica
N1 - Funding Information:
This work was supported by regional funding from NanoCoatings project (POR-FESR 2014-2020, grant number D32I16000040009) as well as by the National Research Council of Italy (ISTEC-CNR). N.M.P. is supported by the European Commission under the Graphene Flagship Core 2 grant number 785219 (WP14 “Composites”) and FET Proactive “Neurofibres” grant number 732344 as well as by the Italian Ministry of Education, University and Research (MIUR) under the “Departments of Excellence” grant L.232/2016 and ARS01-01384-PROSCAN and the PRIN-20177TTP3S.
Funding Information:
This work was supported by regional funding from NanoCoatings project (POR‐FESR 2014‐2020, grant number D32I16000040009) as well as by the National Research Council of Italy (ISTEC‐CNR). N.M.P. is supported by the European Commission under the Graphene Flagship Core 2 grant number 785219 (WP14 “Composites”) and FET Proactive “Neurofibres” grant number 732344 as well as by the Italian Ministry of Education, University and Research (MIUR) under the “Departments of Excellence” grant L.232/2016 and ARS01‐01384‐PROSCAN and the PRIN‐20177TTP3S.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2019/11/30
Y1 - 2019/11/30
N2 - Silks have been widely used as biomaterials due to their biocompatibility, biodegradability, and excellent mechanical properties. In the present work, native spider silk was used as organic template for controlled nucleation of hydroxyapatite (HA) nano-coating that can act as biomimetic interface. Different bio-inspired neutralization methods and process parameters were evaluated to optimize the silk functionalization. The morphology and chemical composition were investigated by scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis and mechanical properties were studied through tensile tests. Results showed that the optimized protocol enabled a controlled and homogeneous nucleation of apatite nano-crystals while maintaining silk mechanical performances after mineralization. This study reports the optimization of a simple and effective bio-inspired nucleation process for precise nucleation of HA onto spider silk templates, suitable to develop high-performance hybrid interfaces for bone tissue engineering.
AB - Silks have been widely used as biomaterials due to their biocompatibility, biodegradability, and excellent mechanical properties. In the present work, native spider silk was used as organic template for controlled nucleation of hydroxyapatite (HA) nano-coating that can act as biomimetic interface. Different bio-inspired neutralization methods and process parameters were evaluated to optimize the silk functionalization. The morphology and chemical composition were investigated by scanning electron microscopy, energy-dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis and mechanical properties were studied through tensile tests. Results showed that the optimized protocol enabled a controlled and homogeneous nucleation of apatite nano-crystals while maintaining silk mechanical performances after mineralization. This study reports the optimization of a simple and effective bio-inspired nucleation process for precise nucleation of HA onto spider silk templates, suitable to develop high-performance hybrid interfaces for bone tissue engineering.
KW - biomimetic
KW - biopolymers and renewable polymers
KW - mechanical properties
KW - surfaces and interfaces
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U2 - 10.1002/app.48739
DO - 10.1002/app.48739
M3 - Article
AN - SCOPUS:85075725312
SN - 0021-8995
VL - 137
JO - Journal of Applied Polymer Science
JF - Journal of Applied Polymer Science
IS - 22
M1 - 48739
ER -