Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

S. Tombelli; M. Ballestri; G. Giambastiani; A. Giannetti; A. Guerrini; G. Sotgiu; C. Trono; G. Tuci; G. Varchi; F. Baldini

doi:10.1117/12.2033185

Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, F. Baldini

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

Original language	English (US)
Title of host publication	European Conference on Biomedical Optics, ECBO 2013
Publisher	Optical Society of America
Volume	8798
ISBN (Print)	9780819496461
DOIs	https://doi.org/10.1117/12.2033185
State	Published - 2013
Event	European Conference on Biomedical Optics, ECBO 2013 - Munich, Germany Duration: May 12 2013 → May 16 2013

Other

Other	European Conference on Biomedical Optics, ECBO 2013
Country/Territory	Germany
City	Munich
Period	5/12/13 → 5/16/13

Keywords

Intracellular sensing
Molecular beacon
Mrna
PMMA nanoparticles
Survivin

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

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10.1117/12.2033185

Cite this

Tombelli, S, Ballestri, M, Giambastiani, G, Giannetti, A, Guerrini, A, Sotgiu, G, Trono, C, Tuci, G, Varchi, G & Baldini, F 2013, Oligonucleotide switches and nanomaterials for intracellular mRNA sensing. in European Conference on Biomedical Optics, ECBO 2013. vol. 8798, 879802, Optical Society of America, European Conference on Biomedical Optics, ECBO 2013, Munich, Germany, 5/12/13. https://doi.org/10.1117/12.2033185

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title = "Oligonucleotide switches and nanomaterials for intracellular mRNA sensing",

abstract = "We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.",

keywords = "Intracellular sensing, Molecular beacon, Mrna, PMMA nanoparticles, Survivin",

author = "S. Tombelli and M. Ballestri and G. Giambastiani and A. Giannetti and A. Guerrini and G. Sotgiu and C. Trono and G. Tuci and G. Varchi and F. Baldini",

year = "2013",

doi = "10.1117/12.2033185",

language = "English (US)",

isbn = "9780819496461",

volume = "8798",

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T1 - Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

AU - Tombelli, S.

AU - Ballestri, M.

AU - Giambastiani, G.

AU - Giannetti, A.

AU - Guerrini, A.

AU - Sotgiu, G.

AU - Trono, C.

AU - Tuci, G.

AU - Varchi, G.

AU - Baldini, F.

PY - 2013

Y1 - 2013

N2 - We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

AB - We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

KW - Intracellular sensing

KW - Molecular beacon

KW - Mrna

KW - PMMA nanoparticles

KW - Survivin

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U2 - 10.1117/12.2033185

DO - 10.1117/12.2033185

M3 - Conference contribution

AN - SCOPUS:84884130725

SN - 9780819496461

VL - 8798

BT - European Conference on Biomedical Optics, ECBO 2013

PB - Optical Society of America

T2 - European Conference on Biomedical Optics, ECBO 2013

Y2 - 12 May 2013 through 16 May 2013

ER -

Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

Abstract

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Keywords

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