@inproceedings{56cab66b6dc647cb8b2edca903cf532c,
title = "Oligonucleotide switches and nanomaterials for intracellular mRNA sensing",
abstract = "We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.",
keywords = "Intracellular sensing, Molecular beacon, Mrna, PMMA nanoparticles, Survivin",
author = "S. Tombelli and M. Ballestri and G. Giambastiani and A. Giannetti and A. Guerrini and G. Sotgiu and C. Trono and G. Tuci and G. Varchi and F. Baldini",
year = "2013",
doi = "10.1117/12.2033185",
language = "English (US)",
isbn = "9780819496461",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "Optical Society of America",
booktitle = "European Conference on Biomedical Optics, ECBO 2013",
note = "European Conference on Biomedical Optics, ECBO 2013 ; Conference date: 12-05-2013 Through 16-05-2013",
}