Oligonucleotide switches and nanomaterials for intracellular mRNA sensing

S. Tombelli, M. Ballestri, G. Giambastiani, A. Giannetti, A. Guerrini, G. Sotgiu, C. Trono, G. Tuci, G. Varchi, F. Baldini

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We describe here the conjugation of polymethylmethacrylate nanoparticles to particular oligonucleotide switches, termed molecular beacons (MBs), as potential intracellular nanosensors. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The nanosensors have been characterized in vitro by investigating the analytical performances of the chosen molecular beacon and its functionalities after conjugation to the nanoparticles.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2013
PublisherOptical Society of America
Volume8798
ISBN (Print)9780819496461
DOIs
StatePublished - 2013
EventEuropean Conference on Biomedical Optics, ECBO 2013 - Munich, Germany
Duration: May 12 2013May 16 2013

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2013
CountryGermany
CityMunich
Period5/12/135/16/13

Keywords

  • Intracellular sensing
  • Molecular beacon
  • Mrna
  • PMMA nanoparticles
  • Survivin

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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