Octachloronaphthalene induction of hepatic microsomal aryl hydrocarbon hydroxylase activity in the immature male rat

Mary Anne Campbell, S. Bandiera, L. Robertson, A. Parkinson, S. Safe

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Administration of octachloronaphthalene to immature male Wistar rats resulted in a dose-dependent increase in several enzymic, electrophoretic and spectral parameters associated with induction of the hepatic microsomal enzymes. Compared to corn-oil (control) treated animals octachloronaphthalene (150 μmol · kg-1 induced hepatic cytochrome P-450 (1.5-fold), benzo [a]pyrene hydroxylase (18-fold) and 4-chlorobiphenyl hydroxylase (18-fold) enzyme activities. In addition to increases in the relative peak intensities of the reduced microsomal cytochrome P-450 : CO and ethylisocyanide (EIC) difference spectra the peak maxima were observed at 448.5 and 452.2/428.0 nm, respectively. The effects of administering octachloronaphthalene to the rat were similar to those observed after pretreatment with 3-methylcholanthrene (MC) and electrophoresis of the induced microsomal proteins showed that both compounds enhanced heme-staining peptides with comparable electrophoretic mobilities. Moreover coadministration of MC (3 × 10 βmol · kg-1) and octachloronaphthalene (2 × 150 μmol · kg-1) indicated that their inductive effects were not additive. It was concluded that octachloronaphthalene was an MC-type inducer of hepatic microsomal enzymes.

Original languageEnglish (US)
Pages (from-to)123-132
Number of pages10
JournalToxicology
Volume22
Issue number2
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Toxicology

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