Nurr1 expression and its modulation in microglia

Xiaolan Fan, Guangrui Luo, Ming Ming, Pu Pu, Liang Li, Dehua Yang, Weidong Le

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Objective: Nurr1, a transcription factor essential for the differentiation and maturation of central dopaminergic cells, is primarily expressed in neurons of the central nervous system. In this study, we intend to determine the expression and modulation of Nurr1 in cultured microglia. Methods: Nurr1 expression in cultured primary mouse microglia was measured by reverse transcription polymerase chain reaction, Western blot and immunofluorescent staining. Lipopolysaccharide (LPS) was used to activate microglia. Specific inhibitors were used to investigate whether the mitogen- activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK), MAPK/Jun N-terminal kinase (JNK), P38 MAPK and phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathways were involved in the modulation of Nurr1 expression. Results: Nurr1 was found to be located at both the cytoplasm and the nucleus of the microglia. After LPS stimulation, the expression of Nurr1 was significantly increased, which could be partially blocked by inhibitors of ERK, JNK and PI3K/Akt, but not by the P38 MAPK inhibitor; the ERK inhibitor can partially block the translocation of Nurr1 from the cytoplasm to the nucleus. Conclusion: Nurr1 is found to be expressed in cultured mouse microglia. The expression of Nurr1 is increased and the protein is translocated from the cytoplasm to the nucleus after activation by LPS, which could be modulated by the ERK, JNK and PI3K/Akt pathways.

Original languageEnglish (US)
Pages (from-to)162-170
Number of pages9
JournalNeuroImmunoModulation
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2009

Keywords

  • Microglia
  • Mitogen-activated protein kinase
  • Nurr1
  • Phosphoinositide 3-kinase/protein kinase B pathway

ASJC Scopus subject areas

  • Endocrinology
  • Immunology
  • Endocrine and Autonomic Systems
  • Neurology

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