TY - JOUR
T1 - Nuclear immobilization of DsRed1 tagged proteins
T2 - A novel tool for studying DNA-protein interactions?
AU - Damdimopoulos, Anastasios E.
AU - Gustafsson, Jan Åke
AU - Spyrou, Giannis
N1 - Funding Information:
This work was supported by the Swedish Research Council Project K99-13X-10370 and Karolinska Institutet.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/6
Y1 - 2007/6
N2 - DsRed1 is a red fluorescent protein that can be used as a fusion partner with other proteins to determine their subcellular localization, similarly to the popular green fluorescent proteins (GFP). Here, we report that fusion of DsRed1 to estrogen receptor α (ERα) renders the transcription factor immobile within the nucleus. Furthermore, we show that the immobilization is dependent on DNA interaction and that the binding to the DNA can be direct as well as indirect for DsRed to immobilize with its fusion partners. This observation could provide a new tool to be used for the identification of target genes containing low affinity binding sites for several transcription factors including ERα. In addition, it could be employed for studies on protein-DNA interactions as well as protein-protein interactions during protein complex formation on chromatin in the event of transcription initiation and regulation.
AB - DsRed1 is a red fluorescent protein that can be used as a fusion partner with other proteins to determine their subcellular localization, similarly to the popular green fluorescent proteins (GFP). Here, we report that fusion of DsRed1 to estrogen receptor α (ERα) renders the transcription factor immobile within the nucleus. Furthermore, we show that the immobilization is dependent on DNA interaction and that the binding to the DNA can be direct as well as indirect for DsRed to immobilize with its fusion partners. This observation could provide a new tool to be used for the identification of target genes containing low affinity binding sites for several transcription factors including ERα. In addition, it could be employed for studies on protein-DNA interactions as well as protein-protein interactions during protein complex formation on chromatin in the event of transcription initiation and regulation.
KW - Chromatin
KW - DNA interactions
KW - DsRed
KW - Estrogen receptor
KW - Nuclear protein mobility
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U2 - 10.1016/j.bbamcr.2007.03.022
DO - 10.1016/j.bbamcr.2007.03.022
M3 - Article
C2 - 17493694
AN - SCOPUS:34249747949
VL - 1773
SP - 687
EP - 690
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
SN - 0167-4889
IS - 6
ER -