Nuclear immobilization of DsRed1 tagged proteins: A novel tool for studying DNA-protein interactions?

Anastasios E. Damdimopoulos, Jan Åke Gustafsson, Giannis Spyrou

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

DsRed1 is a red fluorescent protein that can be used as a fusion partner with other proteins to determine their subcellular localization, similarly to the popular green fluorescent proteins (GFP). Here, we report that fusion of DsRed1 to estrogen receptor α (ERα) renders the transcription factor immobile within the nucleus. Furthermore, we show that the immobilization is dependent on DNA interaction and that the binding to the DNA can be direct as well as indirect for DsRed to immobilize with its fusion partners. This observation could provide a new tool to be used for the identification of target genes containing low affinity binding sites for several transcription factors including ERα. In addition, it could be employed for studies on protein-DNA interactions as well as protein-protein interactions during protein complex formation on chromatin in the event of transcription initiation and regulation.

Original languageEnglish (US)
Pages (from-to)687-690
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1773
Issue number6
DOIs
StatePublished - Jun 2007

Keywords

  • Chromatin
  • DNA interactions
  • DsRed
  • Estrogen receptor
  • Nuclear protein mobility

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biophysics

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