Novel vascular molecule involved in monocyte adhesion to aortic endothelium in models of atherogenesis

Leslie M. McEvoy, Hailing Sun, Philip S. Tsao, John P. Cooke, Judith A. Berliner, Eugene C. Butcher

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Adhesion of monocytes to the endothelium in lesion-prone areas is one of the earliest events in fatty streak formation leading to atherogenesis. The molecular basis of increased monocyte adhesion is not fully characterized. We have identified a novel vascular monocyte adhesion associated protein, VMAP- 1, that plays a role in adhesion of monocytes to activated endothelium. Originally selected for its ability to block binding of a mouse monocyte- like cell line (WEHI78/24) to cytokine- or LPS-stimulated cultured mouse endothelial cells in vitro, anti-VMAP-1 mAb LM151 cross-reacts with rabbit endothelium and blocks binding of human monocytes to cultured rabbit aortic endothelial cells stimulated with minimally modified low density lipoprotein, thought to be a physiologically relevant atherogenic stimulus. Most importantly, LM151 prevents adhesion of normal monocytes and monocytoid cells to intact aortic endothelium from cholesterol fed rabbits in an ex vivo assay. VMAP-1 is a 50-kD protein. Immunohistology of vessels reveals focal constitutive expression in aorta and other large vessels. VMAP-1 is thus a novel vascular adhesion-associated protein that appears to play a critical role in monocyte adhesion to aortic endothelial cells in atherogenesis in vivo.

Original languageEnglish (US)
Pages (from-to)2069-2077
Number of pages9
JournalJournal of Experimental Medicine
Volume185
Issue number12
DOIs
StatePublished - Jun 16 1997

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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