Novel siRNA-based molecular beacons for dual imaging and therapy

Emmanuel Chang, Ming Qiang Zhu, Rebekah Drezek

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Short interfering RNAs (siRNAs) have become a mainstream tool reliably used to study and silence protein expression. We offer a proof-of-principle demonstration that siRNAs may be modified into a siRNA-based molecular beacon that activates upon binding to sequence-specific mRNA in cells while mediating RNA interference. We successfully demonstrate detection and knockdown of telomerase expression in human breast cancer cells. This probe provides a novel look at siRNA target validation that is not currently possible in live cells and holds promising potential in biological applications for disease detection and therapy based on mRNA expression, such as a telomerase-targeted siRNA probe in cancer.

Original languageEnglish (US)
Pages (from-to)422-425
Number of pages4
JournalBiotechnology Journal
Issue number4
StatePublished - Apr 2007

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine


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