TY - JOUR
T1 - Novel Silicon Titanium Diboride Micropatterned Substrates for Cellular Patterning
AU - Friguglietti, Jefferson
AU - Das, Susmi
AU - Le, Phi
AU - Fraga, Daniel
AU - Quintela, Marcos
AU - Gazze, Salvatore A.
AU - McPhail, Darius
AU - Gu, Jianhua
AU - Sabek, Omaima
AU - Gaber, A. Osama
AU - Francis, Lewis W.
AU - Zagozdzon-Wosik, Wanda
AU - Merchant, Fatima A.
N1 - Funding Information:
This work was supported in part by funding to F. Merchant from the University of Houston , Research Seed Grants and High Priority Area Research Seed Grants. We acknowledge Karmul Alam and Kamyar Ahmadi at the University of Houston for their assistance with e-beam evaporation during microfabrication, and in performing OCP measurements, respectively.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/6
Y1 - 2020/6
N2 - Both hard material photolithography and soft lithography are widely used for patterned cell culture. Soft lithography techniques enable bioactive molecule incorporation, however complex surface modifications are required to introduce specific ligands or proteins in conventional photolithography. In this study, we demonstrate human umbilical vein cell (HUVEC) and adult bone marrow derived mesenchymal stem cell (MSC) patterning on titanium diboride (TiB2) layers deposited on silicon (Si) substrates by electron-beam evaporation and micropatterned using photolithography. Micropatterned cell growth specificity on geometric shapes of circle and/or lines is achieved via differential growth factors adsorption in the presence of heparin. Specifically, the deposited films of TiB2 showed increased stiffness, hardness, hydrophilicity and surface charge when compared to background Si. These substrates were found to be compatible with HUVEC and MSC viability, based on biomarker expression and RNA-sequence transcriptome analysis. Cell-type dependent, micropattern selective cell growth, such as contact guidance, alignment, and durotaxis, were observed. In addition, MSC clustering was achieved, enabling a three-dimensional (3D) aggregate based microenvironment during culture. This study clearly demonstrates the potential of microfabricated Si and TiB2 biomaterials for patterned cell culture in vitro, independent of any additional surface modification.
AB - Both hard material photolithography and soft lithography are widely used for patterned cell culture. Soft lithography techniques enable bioactive molecule incorporation, however complex surface modifications are required to introduce specific ligands or proteins in conventional photolithography. In this study, we demonstrate human umbilical vein cell (HUVEC) and adult bone marrow derived mesenchymal stem cell (MSC) patterning on titanium diboride (TiB2) layers deposited on silicon (Si) substrates by electron-beam evaporation and micropatterned using photolithography. Micropatterned cell growth specificity on geometric shapes of circle and/or lines is achieved via differential growth factors adsorption in the presence of heparin. Specifically, the deposited films of TiB2 showed increased stiffness, hardness, hydrophilicity and surface charge when compared to background Si. These substrates were found to be compatible with HUVEC and MSC viability, based on biomarker expression and RNA-sequence transcriptome analysis. Cell-type dependent, micropattern selective cell growth, such as contact guidance, alignment, and durotaxis, were observed. In addition, MSC clustering was achieved, enabling a three-dimensional (3D) aggregate based microenvironment during culture. This study clearly demonstrates the potential of microfabricated Si and TiB2 biomaterials for patterned cell culture in vitro, independent of any additional surface modification.
KW - 3D aggregates
KW - Cellular patterning
KW - Human umbilical vein endothelial cells
KW - Mesenchymal stem cells
KW - Micropatterned substrate
KW - Silicon–titanium diboride substrate
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U2 - 10.1016/j.biomaterials.2020.119927
DO - 10.1016/j.biomaterials.2020.119927
M3 - Article
C2 - 32199283
AN - SCOPUS:85081974446
VL - 244
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
M1 - 119927
ER -