Novel potential ALL low-risk markers revealed by gene expression profiling with new high-throughput SSH-CCS-PCR

J. Qiu, P. Gunaratne, Leif E. Peterson, D. Khurana, N. Walsham, H. Loulseged, R. J. Karni, E. Roussel, R. A. Gibs, J. F. Margolin, M. C. Gingras

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The current systems of risk grouping in pediatric acute lymphoblastic leukemia (ALL) fail to predict therapeutic success in 10-35% of patients. To identify better predictive markers of clinical behavior in ALL, we have developed an integrated approach for gene expression profiling that couples suppression subtractive hybridization, concatenated cDNA sequencing, and reverse transcriptase real-time quantitative PCR. Using this approach, a total of 600 differentially expressed genes were identified between t(4;11) ALL and pre-B ALL with no determinant chromosomal translocation. The expression of 67 genes was analyzed in different cytogenetic ALL subgroups and B lymphocytes isolated from healthy donors. Three genes, BACH1, TP53BPL, and H2B/S, were consistently expressed as a significant cluster associated with the low-risk ALL subgroups. A total of 42 genes were differentially expressed in ALL vs normal B lymphocytes, with no specific association with any particular ALL subgroups. The remaining 22 genes were part of a specific expression profile associated with the hyperdiploid, t(12;21), or t(4;11) subgroups. Using an unsupervised hierarchical cluster analysis, the discriminating power of these specific expression profiles allowed the clustering of patients according to their subgroups. These genes could help to understand the difference in treatment response and become therapeutical targets to improve ALL clinical outcomes.

Original languageEnglish (US)
Pages (from-to)1891-1900
Number of pages10
JournalLeukemia
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2003

Keywords

  • Acute lymphoblastic leukemia
  • BACH1
  • Gene expression profiling
  • H2B/S
  • Suppression subtractive hybridization/concatenated cDNA sequencing/reverse transcriptase real-time quantitative PCR
  • TP53BPL

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Fingerprint Dive into the research topics of 'Novel potential ALL low-risk markers revealed by gene expression profiling with new high-throughput SSH-CCS-PCR'. Together they form a unique fingerprint.

Cite this