TY - JOUR
T1 - Novel peptide ligands that bind specifically to mouse embryonic stem cells
AU - Zhao, Saijuan
AU - Zhao, Wenxiu
AU - Ma, Lan
N1 - Funding Information:
Financial support provided by the Hi-tech Research and Development Program of China (No. 2006AA03Z359 ) is gratefully acknowledged. We would also like to thank Ni Hu, Jingfang Zhang, and Lei Li for their technical assistance. We are also thankful to The Joint Lab of Stem Cell Research for supplying the mouse embryonic stem cells.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/11
Y1 - 2010/11
N2 - The search for new ES cell markers is critical not only for identification, isolation and visualization of embryonic stem (ES) cells, but also for potential clinical treatment as a targeting agent. Here, by using phage display technology, 12-mer peptide ligands that bind specifically to mouse ES cells were isolated. After four rounds of negative-positive selection, nine sequences in 20 random samples from the chosen clones were selected. Enzyme-linked immunosorbent assay results suggested the Seq2 peptide (KHMHWHPPALNT) had high affinity and specificity to the mouse ES cells. The binding capability of the Seq2 phage could be matched with that of a chemically synthesized peptide with a sequence identical to that displayed by the phage, indicating that this ability was due to the peptide sequence itself. Immunofluorescence analysis confirmed that Seq2 phage selectively bound to the mouse ES cells but not to the differentiated mouse ES cells. Western blot analysis proved the Seq2 phage was bound to two mouse ES membrane proteins which were about 18/20KD, suggesting that the selected peptide targeted to a unique receptor expressed on the mouse ES cells with specificity. Peptides obtained from the study may provide a way to label, identify, and characterize ES cells.
AB - The search for new ES cell markers is critical not only for identification, isolation and visualization of embryonic stem (ES) cells, but also for potential clinical treatment as a targeting agent. Here, by using phage display technology, 12-mer peptide ligands that bind specifically to mouse ES cells were isolated. After four rounds of negative-positive selection, nine sequences in 20 random samples from the chosen clones were selected. Enzyme-linked immunosorbent assay results suggested the Seq2 peptide (KHMHWHPPALNT) had high affinity and specificity to the mouse ES cells. The binding capability of the Seq2 phage could be matched with that of a chemically synthesized peptide with a sequence identical to that displayed by the phage, indicating that this ability was due to the peptide sequence itself. Immunofluorescence analysis confirmed that Seq2 phage selectively bound to the mouse ES cells but not to the differentiated mouse ES cells. Western blot analysis proved the Seq2 phage was bound to two mouse ES membrane proteins which were about 18/20KD, suggesting that the selected peptide targeted to a unique receptor expressed on the mouse ES cells with specificity. Peptides obtained from the study may provide a way to label, identify, and characterize ES cells.
KW - Cell-specific peptides
KW - Differentiated cells
KW - Mouse embryonic stem cells
KW - Phage-displayed peptide library screening
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U2 - 10.1016/j.peptides.2010.08.004
DO - 10.1016/j.peptides.2010.08.004
M3 - Article
C2 - 20713104
AN - SCOPUS:77957837401
VL - 31
SP - 2027
EP - 2034
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 11
ER -