TY - JOUR
T1 - Novel amphiphilic triblock copolymer based on PPDO, PCL, and PEG
T2 - Synthesis, characterization, and aqueous dispersion
AU - Remant Bahadur, K. C.
AU - Bhattarai, Shanta Raj
AU - Aryal, Santosh
AU - Khil, Myung Seob
AU - Dharmaraj, N.
AU - Kim, Hak Yong
N1 - Funding Information:
This work was supported by the Korean Research Foundation (KRF) Grant Funded by Korean Government (MOEHRD) and Regional Research Centers Program of the Korean Ministry of Educational and Human Resources Development through the Center for Healthcare Technology Development, Chonbuk National University, Jeonju 561-756, Republic of Korea.
PY - 2007/1/5
Y1 - 2007/1/5
N2 - Amphiphilic triblock copolymer, poly(p-dioxanone-co-caprolactone)-block-poly(ethylene oxide)-block-poly(p-dioxanone-co-caprolactone) (PPDO-co-PCL-b-PEO-b-PPDO-co-PCL) was synthesized by ring opening polymerization (ROP) of p-dioxanone and ε-caprolactone initiated through the hydroxyl end of poly(ethylene glycol) (PEG) in the presence of stannous 2-ethyl hexanoate [Sn(oct)2] as a catalyst. Polymerization and structural features of the polymers were analyzed by different physicochemical techniques (GPC, 1H NMR, 13C NMR, FT-IR, DSC and TGA). The splitting of 1H NMR resonance at δ 2.3 and δ 4.1 ppm reveals the random copolymerization. Polymeric nanoparticles were prepared in phosphate buffer (pH 7.4) by co-solvent evaporation technique at room temperature (25 °C). Existence of hydrophobic domains as cores of the micelles were characterized by 1H NMR spectroscopy and further confirmed with fluorescence technique using pyrene as a probe. Critical micelle concentration (CMC) of the polymer in phosphate buffer (pH. 7.4) was decreased from 2.3 × 10-3 to 7.6 × 10-4 g/L with the fraction of PCL. Polymeric nanoparticles observed by atomic force microscopy (AFM) were uniform and spherical, with smooth textured of around 50-30 nm diameter. Dynamic light scattering (DLS) and electrophoretic light scattering (ELS) measurements showed a monodisperse size distribution of around 113-90 nm hydrodynamic diameters and negative zeta (ζ) potential (-4 to -14 mV), respectively. The investigations for the polymeric nanoparticles in aqueous medium showed that the composition of the hydrophobic segment of amphiphilic block copolymer makes a significant influence on its physicochemical characteristics.
AB - Amphiphilic triblock copolymer, poly(p-dioxanone-co-caprolactone)-block-poly(ethylene oxide)-block-poly(p-dioxanone-co-caprolactone) (PPDO-co-PCL-b-PEO-b-PPDO-co-PCL) was synthesized by ring opening polymerization (ROP) of p-dioxanone and ε-caprolactone initiated through the hydroxyl end of poly(ethylene glycol) (PEG) in the presence of stannous 2-ethyl hexanoate [Sn(oct)2] as a catalyst. Polymerization and structural features of the polymers were analyzed by different physicochemical techniques (GPC, 1H NMR, 13C NMR, FT-IR, DSC and TGA). The splitting of 1H NMR resonance at δ 2.3 and δ 4.1 ppm reveals the random copolymerization. Polymeric nanoparticles were prepared in phosphate buffer (pH 7.4) by co-solvent evaporation technique at room temperature (25 °C). Existence of hydrophobic domains as cores of the micelles were characterized by 1H NMR spectroscopy and further confirmed with fluorescence technique using pyrene as a probe. Critical micelle concentration (CMC) of the polymer in phosphate buffer (pH. 7.4) was decreased from 2.3 × 10-3 to 7.6 × 10-4 g/L with the fraction of PCL. Polymeric nanoparticles observed by atomic force microscopy (AFM) were uniform and spherical, with smooth textured of around 50-30 nm diameter. Dynamic light scattering (DLS) and electrophoretic light scattering (ELS) measurements showed a monodisperse size distribution of around 113-90 nm hydrodynamic diameters and negative zeta (ζ) potential (-4 to -14 mV), respectively. The investigations for the polymeric nanoparticles in aqueous medium showed that the composition of the hydrophobic segment of amphiphilic block copolymer makes a significant influence on its physicochemical characteristics.
KW - Biomaterials
KW - Block copolymers
KW - Micelles
KW - Nanoparticles
KW - Self-assembled
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U2 - 10.1016/j.colsurfa.2006.06.009
DO - 10.1016/j.colsurfa.2006.06.009
M3 - Article
AN - SCOPUS:33845521649
VL - 292
SP - 69
EP - 78
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
SN - 0927-7757
IS - 1
ER -