Notch 1 signaling regulates peripheral T cell activation

Research output: Contribution to journalArticle

Todd N. Eagar, Qizhi Tang, Michael Wolfe, Yiping He, Warren S. Pear, Jeffrey A. Bluestone

Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3β phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.

Original languageEnglish (US)
Pages (from-to)407-415
Number of pages9
JournalImmunity
Volume20
Issue number4
DOIs
StatePublished - Apr 1 2004

PMID: 15084270

Altmetrics

Cite this

Standard

Notch 1 signaling regulates peripheral T cell activation. / Eagar, Todd N.; Tang, Qizhi; Wolfe, Michael; He, Yiping; Pear, Warren S.; Bluestone, Jeffrey A.

In: Immunity, Vol. 20, No. 4, 01.04.2004, p. 407-415.

Research output: Contribution to journalArticle

Harvard

Eagar, TN, Tang, Q, Wolfe, M, He, Y, Pear, WS & Bluestone, JA 2004, 'Notch 1 signaling regulates peripheral T cell activation' Immunity, vol. 20, no. 4, pp. 407-415. https://doi.org/10.1016/S1074-7613(04)00081-0

APA

Eagar, T. N., Tang, Q., Wolfe, M., He, Y., Pear, W. S., & Bluestone, J. A. (2004). Notch 1 signaling regulates peripheral T cell activation. Immunity, 20(4), 407-415. https://doi.org/10.1016/S1074-7613(04)00081-0

Vancouver

Eagar TN, Tang Q, Wolfe M, He Y, Pear WS, Bluestone JA. Notch 1 signaling regulates peripheral T cell activation. Immunity. 2004 Apr 1;20(4):407-415. https://doi.org/10.1016/S1074-7613(04)00081-0

Author

Eagar, Todd N. ; Tang, Qizhi ; Wolfe, Michael ; He, Yiping ; Pear, Warren S. ; Bluestone, Jeffrey A. / Notch 1 signaling regulates peripheral T cell activation. In: Immunity. 2004 ; Vol. 20, No. 4. pp. 407-415.

BibTeX

@article{0fc5eae7afb647499f4bc6eae83e8d00,
title = "Notch 1 signaling regulates peripheral T cell activation",
abstract = "Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3β phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.",
author = "Eagar, {Todd N.} and Qizhi Tang and Michael Wolfe and Yiping He and Pear, {Warren S.} and Bluestone, {Jeffrey A.}",
year = "2004",
month = "4",
day = "1",
doi = "10.1016/S1074-7613(04)00081-0",
language = "English (US)",
volume = "20",
pages = "407--415",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Notch 1 signaling regulates peripheral T cell activation

AU - Eagar, Todd N.

AU - Tang, Qizhi

AU - Wolfe, Michael

AU - He, Yiping

AU - Pear, Warren S.

AU - Bluestone, Jeffrey A.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3β phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.

AB - Notch signaling has been identified as an important regulator of leukocyte differentiation and thymic maturation. Less is known about the role of Notch signaling in regulating mature T cells. We examined the role of Notch 1 in regulating peripheral T cell activity in vitro and in vivo. Coligation of Notch 1 together with TCR and CD28 resulted in a dramatic inhibition of T cell activation, proliferation, and cytokine production. This effect was dependent on presenilin activity and induced the expression of HES-1, suggestive of Notch 1 signaling. Biochemical analysis demonstrated an inhibition of AKT and GSK3β phosphorylation following Notch 1 engagement while other biochemical signals such as TCR and ERK phosphorylation remained intact. Similar effects were observed in vivo in an adoptive transfer model. Therefore, Notch 1 signaling may play an important role in regulating naive T cell activation and homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=1842785211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842785211&partnerID=8YFLogxK

U2 - 10.1016/S1074-7613(04)00081-0

DO - 10.1016/S1074-7613(04)00081-0

M3 - Article

VL - 20

SP - 407

EP - 415

JO - Immunity

T2 - Immunity

JF - Immunity

SN - 1074-7613

IS - 4

ER -

ID: 16789308