NOx and ADMA changes with focal ischemia, amelioration with the chaperonin GroEL

Lijun Xu; Bingyin Wang; Kirandeep Kaur; Melanie F. Kho; John P. Cooke; Rona G. Giffard

doi:10.1016/j.neulet.2007.03.021

NO_x and ADMA changes with focal ischemia, amelioration with the chaperonin GroEL

Lijun Xu, Bingyin Wang, Kirandeep Kaur, Melanie F. Kho, John P. Cooke, Rona G. Giffard

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Both nitric oxide and asymmetric dimethylarginine (ADMA) play a critical role in the regulation of cerebral blood flow, though their neuroprotective and cytotoxic effects are still under investigation. In this study, we found that nitrate/nitrite (NO_x) levels in plasma, ischemic brain tissue, and cerebrospinal fluid (CSF) increased significantly 24 h after 2 h transient middle cerebral artery occlusion (MCAO) in rats. ADMA levels were unchanged in plasma, but decreased significantly in CSF 24 h following MCAO. The CSF ADMA/NO_x ratio decreased markedly following ischemia. Rats protected by expression of the chaperonin GroEL or its folding deficient mutant D87K had lower plasma NO_x levels at 24 h reperfusion. ADMA, NO, and their ratio in CSF merit further study as biomarkers for ischemic brain injury.

Original language	English (US)
Pages (from-to)	201-204
Number of pages	4
Journal	Neuroscience Letters
Volume	418
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2007.03.021
State	Published - May 17 2007

Keywords

ADMA
CSF
Focal ischemia
GroEL
NO
Rat

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/j.neulet.2007.03.021

Cite this

@article{010dc085195b481fb7202e4de561201e,

title = "NOx and ADMA changes with focal ischemia, amelioration with the chaperonin GroEL",

abstract = "Both nitric oxide and asymmetric dimethylarginine (ADMA) play a critical role in the regulation of cerebral blood flow, though their neuroprotective and cytotoxic effects are still under investigation. In this study, we found that nitrate/nitrite (NOx) levels in plasma, ischemic brain tissue, and cerebrospinal fluid (CSF) increased significantly 24 h after 2 h transient middle cerebral artery occlusion (MCAO) in rats. ADMA levels were unchanged in plasma, but decreased significantly in CSF 24 h following MCAO. The CSF ADMA/NOx ratio decreased markedly following ischemia. Rats protected by expression of the chaperonin GroEL or its folding deficient mutant D87K had lower plasma NOx levels at 24 h reperfusion. ADMA, NO, and their ratio in CSF merit further study as biomarkers for ischemic brain injury.",

keywords = "ADMA, CSF, Focal ischemia, GroEL, NO, Rat",

author = "Lijun Xu and Bingyin Wang and Kirandeep Kaur and Kho, {Melanie F.} and Cooke, {John P.} and Giffard, {Rona G.}",

note = "Funding Information: Dr. Cooke is the inventor of patents owned by Stanford University for diagnostic and therapeutic applications of the NOS pathway from which he receives royalties. Sponsorship: Supported in part by NIH grants GM 49831 and NS 37520 to RGG and HL-63685, HL-75774, CA098303 to JPC, and the Tobacco Related Disease Research Program Grant 11RT-0147 to JPC. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2007",

month = may,

day = "17",

doi = "10.1016/j.neulet.2007.03.021",

language = "English (US)",

volume = "418",

pages = "201--204",

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AU - Wang, Bingyin

AU - Kaur, Kirandeep

AU - Kho, Melanie F.

AU - Cooke, John P.

AU - Giffard, Rona G.

N1 - Funding Information: Dr. Cooke is the inventor of patents owned by Stanford University for diagnostic and therapeutic applications of the NOS pathway from which he receives royalties. Sponsorship: Supported in part by NIH grants GM 49831 and NS 37520 to RGG and HL-63685, HL-75774, CA098303 to JPC, and the Tobacco Related Disease Research Program Grant 11RT-0147 to JPC. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2007/5/17

Y1 - 2007/5/17

N2 - Both nitric oxide and asymmetric dimethylarginine (ADMA) play a critical role in the regulation of cerebral blood flow, though their neuroprotective and cytotoxic effects are still under investigation. In this study, we found that nitrate/nitrite (NOx) levels in plasma, ischemic brain tissue, and cerebrospinal fluid (CSF) increased significantly 24 h after 2 h transient middle cerebral artery occlusion (MCAO) in rats. ADMA levels were unchanged in plasma, but decreased significantly in CSF 24 h following MCAO. The CSF ADMA/NOx ratio decreased markedly following ischemia. Rats protected by expression of the chaperonin GroEL or its folding deficient mutant D87K had lower plasma NOx levels at 24 h reperfusion. ADMA, NO, and their ratio in CSF merit further study as biomarkers for ischemic brain injury.

AB - Both nitric oxide and asymmetric dimethylarginine (ADMA) play a critical role in the regulation of cerebral blood flow, though their neuroprotective and cytotoxic effects are still under investigation. In this study, we found that nitrate/nitrite (NOx) levels in plasma, ischemic brain tissue, and cerebrospinal fluid (CSF) increased significantly 24 h after 2 h transient middle cerebral artery occlusion (MCAO) in rats. ADMA levels were unchanged in plasma, but decreased significantly in CSF 24 h following MCAO. The CSF ADMA/NOx ratio decreased markedly following ischemia. Rats protected by expression of the chaperonin GroEL or its folding deficient mutant D87K had lower plasma NOx levels at 24 h reperfusion. ADMA, NO, and their ratio in CSF merit further study as biomarkers for ischemic brain injury.

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