NOx and ADMA changes with focal ischemia, amelioration with the chaperonin GroEL

Lijun Xu, Bingyin Wang, Kirandeep Kaur, Melanie F. Kho, John P. Cooke, Rona G. Giffard

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Both nitric oxide and asymmetric dimethylarginine (ADMA) play a critical role in the regulation of cerebral blood flow, though their neuroprotective and cytotoxic effects are still under investigation. In this study, we found that nitrate/nitrite (NOx) levels in plasma, ischemic brain tissue, and cerebrospinal fluid (CSF) increased significantly 24 h after 2 h transient middle cerebral artery occlusion (MCAO) in rats. ADMA levels were unchanged in plasma, but decreased significantly in CSF 24 h following MCAO. The CSF ADMA/NOx ratio decreased markedly following ischemia. Rats protected by expression of the chaperonin GroEL or its folding deficient mutant D87K had lower plasma NOx levels at 24 h reperfusion. ADMA, NO, and their ratio in CSF merit further study as biomarkers for ischemic brain injury.

Original languageEnglish (US)
Pages (from-to)201-204
Number of pages4
JournalNeuroscience Letters
Volume418
Issue number2
DOIs
StatePublished - May 17 2007

Keywords

  • ADMA
  • CSF
  • Focal ischemia
  • GroEL
  • NO
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

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