TY - JOUR
T1 - Normothermic renal artery perfusion
T2 - A comparison of perfusates
AU - Hughes, John D.
AU - Chen, Changyi
AU - Mattar, Samer G.
AU - Someren, Ayten
AU - Noe, Beverly
AU - Suwyn, Carolyn R.
AU - Lumsden, Alan B.
PY - 1996/3
Y1 - 1996/3
N2 - Hypothermia and preservative perfusates have been used to decrease ischemic renal injury. This study was performed to identify the preservative function of perfusates independent of the effects of hypothermia. Rats underwent 45 minutes of renal ischemia. Rectal and renal parenchyma temperatures were monitored and maintained within 1° C of normal. Perfusates were University of Wisconsin solution (UW), Euro-Collins solution, normal saline solution, and Ringer's lactate solution. A nonperfused ischemic control and a nonischemic control group were also evaluated. Parameters evaluated included serum creatinine and blood urea nitrogen levels, renal ischemic injury grade, renal weight, and gross appearance of the injured kidney. Rats treated with UW solution were found to have a significantly lower creatinine, blood urea nitrogen, and injury grade than the other three perfused groups. The external gross appearance of the UW-treated kidneys was normal, whereas that of the other groups demonstrated moderate to severe injury. Although the mean right/left renal weight difference of the UW- treated group was lower than that of the other three groups, this was not statistically significant. Under normothermic conditions in rats, UW solution affords significant renal protection from ischemia, Euro-Collins, normal saline, and Ringer's lactate solutions display no significant protective effect.
AB - Hypothermia and preservative perfusates have been used to decrease ischemic renal injury. This study was performed to identify the preservative function of perfusates independent of the effects of hypothermia. Rats underwent 45 minutes of renal ischemia. Rectal and renal parenchyma temperatures were monitored and maintained within 1° C of normal. Perfusates were University of Wisconsin solution (UW), Euro-Collins solution, normal saline solution, and Ringer's lactate solution. A nonperfused ischemic control and a nonischemic control group were also evaluated. Parameters evaluated included serum creatinine and blood urea nitrogen levels, renal ischemic injury grade, renal weight, and gross appearance of the injured kidney. Rats treated with UW solution were found to have a significantly lower creatinine, blood urea nitrogen, and injury grade than the other three perfused groups. The external gross appearance of the UW-treated kidneys was normal, whereas that of the other groups demonstrated moderate to severe injury. Although the mean right/left renal weight difference of the UW- treated group was lower than that of the other three groups, this was not statistically significant. Under normothermic conditions in rats, UW solution affords significant renal protection from ischemia, Euro-Collins, normal saline, and Ringer's lactate solutions display no significant protective effect.
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U2 - 10.1007/BF02000755
DO - 10.1007/BF02000755
M3 - Article
C2 - 8733863
AN - SCOPUS:0029930298
SN - 0890-5096
VL - 10
SP - 123
EP - 130
JO - Annals of Vascular Surgery
JF - Annals of Vascular Surgery
IS - 2
ER -