Normal forebrain development may require continual Wnt antagonism until mid-somitogenesis in zebrafish

Jun Dae Kim, Hang Suk Chun, Seok Hyung Kim, Hyung Seok Kim, Young Seop Kim, Myoung Jin Kim, Jimann Shin, Myungchull Rhee, Sang Yeob Yeo, Tae Lin Huh

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


During normal forebrain development in vertebrates, rostral neural tissue must be protected from Wnt signals via the actions of locally expressed Wnt antagonistic factors. In zebrafish zygotic oep (Zoep) mutants, forebrain structure is severely disrupted with reduced expression of the Wnt antagonists secreted frizzled related protein1 and dickkopf1. To analyze the temporal effects of Wnt antagonism on forebrain development, we generated transgenic zebrafish that overexpressed the dominant negative form of frizzled8a (DNfz8a) in wild-type and Zoep mutants under the control of a heat-inducible promoter. This model allowed for assessment of the dynamics of Wnt antagonistic signaling during forebrain development. Our results demonstrated that overexpression of DNfz8a in Zoep embryos between 7 and 16 hpf increased putative forebrain region demarcated by anf and distal-less2 expressions. These results suggest that normal forebrain development requires continual Wnt antagonism from the early gastrula to the mid-somitogenesis stage.

Original languageEnglish (US)
Pages (from-to)717-721
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number4
StatePublished - Apr 17 2009


  • Forebrain development
  • Transgenic zebrafish
  • Wnt antagonist
  • Zygotic one-eyed pinhead (oep) mutant
  • frizzled8a

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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