TY - JOUR
T1 - Nonmammalian model systems to investigate fungal biofilms
AU - Arvanitis, Marios
AU - Fuchs, Beth Burgwyn
AU - Mylonakis, Eleftherios
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
PY - 2014
Y1 - 2014
N2 - Medical advances have resulted in an increase in the number of patients in immunocompromised states, vulnerable to infection, or individuals fitted with medical devices that form niches for microbial infections. These infections are difficult to treat and have significant morbidity and mortality rates. An important factor in the pathogenesis of fungal diseases is the development of biofilm-forming communities, enabling the invasion of host tissues and resistance to antimicrobial compounds. To investigate the genetic requirements for filamentation and seek compounds that inhibit the process, invertebrate hosts are employed as models of in vivo infection. The purpose of our review is to highlight methods that can be utilized to investigate fungal filamentation, an important step in the development of biofilms, in the invertebrate hosts Galleria mellonella, Caenorhabditis elegans , and Drosophila melanogaster.
AB - Medical advances have resulted in an increase in the number of patients in immunocompromised states, vulnerable to infection, or individuals fitted with medical devices that form niches for microbial infections. These infections are difficult to treat and have significant morbidity and mortality rates. An important factor in the pathogenesis of fungal diseases is the development of biofilm-forming communities, enabling the invasion of host tissues and resistance to antimicrobial compounds. To investigate the genetic requirements for filamentation and seek compounds that inhibit the process, invertebrate hosts are employed as models of in vivo infection. The purpose of our review is to highlight methods that can be utilized to investigate fungal filamentation, an important step in the development of biofilms, in the invertebrate hosts Galleria mellonella, Caenorhabditis elegans , and Drosophila melanogaster.
KW - Biofilm
KW - Caenorhabditis elegans
KW - Drosophila melanogaster
KW - Filamentation
KW - Fungal infection
KW - Galleria mellonella
UR - http://www.scopus.com/inward/record.url?scp=84910117332&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-0467-9_11
DO - 10.1007/978-1-4939-0467-9_11
M3 - Article
C2 - 24664832
AN - SCOPUS:84910117332
SN - 1064-3745
VL - 1147
SP - 159
EP - 172
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -