Abstract
Objective: Prenatal noninvasive determination of fetal Rh status is an important aid to the management of hemolytic disease of the fetus and newborn. We performed real-time polymerase chain reaction on fetal DNA derived from maternal plasma to determine fetal Rh status. Study design: Cell-free plasma DNA from 98 D-negative pregnant women was tested for the presence of exons 4, 5, and 10 of RHD. The presence of fetal DNA was confirmed by detection of SRY or biallelic insertion/deletion polymorphisms in the maternal plasma and buffy coat. Results: Seventy-two D-positive infants and 26 D-negative infants were determined by serologic studies. All 3 RHD exon sequences were detected in 68 of 72 mothers of D-positive infants. The presence of fetal DNA in mothers of D-negative infants was confirmed in all 10 boys and in 14 of 16 girls. Conclusion: Fetal RHD genotyping in this study correctly predicted fetal Rh status in 92 of 98 (94%) cases.
Original language | English (US) |
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Pages (from-to) | 1966-1971 |
Number of pages | 6 |
Journal | American Journal of Obstetrics and Gynecology |
Volume | 193 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2005 |
Keywords
- Fetal DNA in maternal plasma
- Prenatal RHD genotyping
- Real-time polymerase chain reaction
ASJC Scopus subject areas
- Obstetrics and Gynecology