TY - JOUR
T1 - Noninvasive indexes of cardiac systolic and diastolic function in hyperthyroid and senescent mouse
AU - Taffet, George
AU - Hartley, Craig J.
AU - Wen, Xiouying
AU - Pham, Thuy
AU - Michael, Lloyd H.
AU - Entman, Mark L.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1996/6
Y1 - 1996/6
N2 - The mouse is a common model for transgenic manipulation, however, their small size has made hemodynamic study difficult. A noninvasive 10-MHz pulsed Doppler probe was used to measure aortic and mitral flow velocities in anesthetized, intact mice to study the effects of aging and hyperthyroidism (induced by thyroxine) on systolic and diastolic cardiac function. In 10 hyperthyroid mice peak aortic velocity (PAV, an index of systolic function) was 34% higher than in 10 control mice (108 ± 2 vs. 80 ± 3 cm/s, P < 0.05). The ratio of early to late mitral filling velocity (E/A ratio, an index of diastolic function) was 47% higher (5.6 ± 0.8 vs. 3.8 ± 0.2, P < 0.05) in the hyperthyroid mice. In six old (30 mo) mice PAV was similar to eight young (4 mo) mice (73 ± 3 vs. 75 ± 3 cm/s), but the E/A ratio was 59% lower (1.8 ± 0.3 vs. 4.4 ± 0.4, P < 0.05). Despite a wide range of observed heart rates, the systolic and diastolic parameters of the groups were clearly separated. We conclude that cardiac systolic and diastolic function in mice, measured by pulsed Doppler ultrasound, are similar to larger species both in magnitude and in their response to hyperthyroidism and aging.
AB - The mouse is a common model for transgenic manipulation, however, their small size has made hemodynamic study difficult. A noninvasive 10-MHz pulsed Doppler probe was used to measure aortic and mitral flow velocities in anesthetized, intact mice to study the effects of aging and hyperthyroidism (induced by thyroxine) on systolic and diastolic cardiac function. In 10 hyperthyroid mice peak aortic velocity (PAV, an index of systolic function) was 34% higher than in 10 control mice (108 ± 2 vs. 80 ± 3 cm/s, P < 0.05). The ratio of early to late mitral filling velocity (E/A ratio, an index of diastolic function) was 47% higher (5.6 ± 0.8 vs. 3.8 ± 0.2, P < 0.05) in the hyperthyroid mice. In six old (30 mo) mice PAV was similar to eight young (4 mo) mice (73 ± 3 vs. 75 ± 3 cm/s), but the E/A ratio was 59% lower (1.8 ± 0.3 vs. 4.4 ± 0.4, P < 0.05). Despite a wide range of observed heart rates, the systolic and diastolic parameters of the groups were clearly separated. We conclude that cardiac systolic and diastolic function in mice, measured by pulsed Doppler ultrasound, are similar to larger species both in magnitude and in their response to hyperthyroidism and aging.
KW - Aging
KW - Early peak-to-late peak ratio
KW - Sarcoplasmic reticulum
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U2 - 10.1152/ajpheart.1996.270.6.h2204
DO - 10.1152/ajpheart.1996.270.6.h2204
M3 - Article
C2 - 8764275
AN - SCOPUS:33750837558
SN - 0363-6135
VL - 270
SP - H2204-H2209
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 6 39-6
ER -