TY - JOUR
T1 - Noninvasive Detection of Inflammatory Changes in White Adipose Tissue by Label-Free Raman Spectroscopy
AU - Haka, Abigail S.
AU - Sue, Erika
AU - Zhang, Chi
AU - Bhardwaj, Priya
AU - Sterling, Joshua
AU - Carpenter, Cassidy
AU - Leonard, Madeline
AU - Manzoor, Maryem
AU - Walker, Jeanne
AU - Aleman, Jose O.
AU - Gareau, Daniel
AU - Holt, Peter R.
AU - Breslow, Jan L.
AU - Zhou, Xi Kathy
AU - Giri, Dilip
AU - Morrow, Monica
AU - Iyengar, Neil
AU - Barman, Ishan
AU - Hudis, Clifford A.
AU - Dannenberg, Andrew J.
N1 - Funding Information:
This work was supported by National Institutes of Health (NIH) (NIH Grant No. UL1TR000457 of the Clinical and Translational Science Center at Weill Cornell Medical College, NIH Grant No. 5RO1 HL093324-07, NIH Grant No. 5P41EB015871-28 of the Laser Biomedical Research Center at the Massachusetts Institute of Technology), as well as the National Center for Advancing Translational Sciences (NCATS) (Grant No. UL1TR000043), the NIH Clinical and Translations Science Award (CTSA) Program at Rockefeller University, and cosponsored by the Center for Basic and Translational Research on Disorders of the Digestive System at Rockefeller University through the generosity of Leona M. and Harry B. Helmsley Charitable Trust, the Sackler Center for Biomedicine and Nutrition through the generosity of the Sackler Foundation, the Botwinick-Wolfensohn Foundation (in memory of Mr. and Mrs. Benjamin Botwinick), and the Breast Cancer Research Foundation.
Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/2/16
Y1 - 2016/2/16
N2 - White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.
AB - White adipose tissue inflammation (WATi) has been linked to the pathogenesis of obesity-related diseases, including type 2 diabetes, cardiovascular disease, and cancer. In addition to the obese, a substantial number of normal and overweight individuals harbor WATi, putting them at increased risk for disease. We report the first technique that has the potential to detect WATi noninvasively. Here, we used Raman spectroscopy to detect WATi with excellent accuracy in both murine and human tissues. This is a potentially significant advance over current histopathological techniques for the detection of WATi, which rely on tissue excision and, therefore, are not practical for assessing disease risk in the absence of other identifying factors. Importantly, we show that noninvasive Raman spectroscopy can diagnose WATi in mice. Taken together, these results demonstrate the potential of Raman spectroscopy to provide objective risk assessment for future cardiometabolic complications in both normal weight and overweight/obese individuals.
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U2 - 10.1021/acs.analchem.5b03696
DO - 10.1021/acs.analchem.5b03696
M3 - Article
C2 - 26752499
AN - SCOPUS:84958259589
VL - 88
SP - 2140
EP - 2148
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 4
ER -