TY - JOUR
T1 - Noninvasive blood pressure measurement in mice using pulsed Doppler ultrasound
AU - Reddy, Anilkumar K.
AU - Taffet, George
AU - Madala, Sridhar
AU - Michael, Lloyd H.
AU - Entman, Mark L.
AU - Hartley, Craig J.
N1 - Funding Information:
The authors acknowledge the contributions of Allen Jones and Christian Martono of Indus Instruments for helping with data-acquisition software, Richard Kwun, Maria Theresa Treviño, and Jennifer Pocius for helping with arterial cannulations, Thuy Pham for help with data acquisition, Kurt Schillinger for providing mice for repeatability studies and James Brooks for editorial review. This work was supported in part by the National Institutes of Health (grants HL-52364, HL-22512, AG-13251), and the Texas Advanced Technology Program.
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Existing tail-cuff pressure devices for mice use tail flow sensors that measure only systolic and mean pressure. We developed a method to obtain systolic and diastolic pressure in mice using a pulsed Doppler flow velocity sensor and a tail-cuff and validated the method against pressure signals obtained simultaneously from a fluid-filled catheter. The tail-cuff was pressurized to suprasystolic levels to completely occlude the tail artery and then released gradually. The pressure at which the tail flow reappeared was recorded as systolic and the pressure at which the tail flow became continuous was recorded as diastolic. Regression analysis of tail-cuff pressures over catheter pressures obtained from healthy mice (n = 16) showed a high degree of association (rsys = 0.95, rdia = 0.94, both at p < 0.001). Bland-Altman analysis showed good agreement between the two methods, with a mean difference of -13 ( ± 12 SD) mmHg and 3 ( ± 10 SD) mmHg in the systolic (58 to 250 mmHg) and diastolic (48 to 178 mmHg) pressure measurements, respectively. Bland-Altman plots of tail-cuff blood pressures of a second group of mice (n = 20) showed good agreement between repeated measurements obtained on the same day, but had higher variability between measurements made on different days.
AB - Existing tail-cuff pressure devices for mice use tail flow sensors that measure only systolic and mean pressure. We developed a method to obtain systolic and diastolic pressure in mice using a pulsed Doppler flow velocity sensor and a tail-cuff and validated the method against pressure signals obtained simultaneously from a fluid-filled catheter. The tail-cuff was pressurized to suprasystolic levels to completely occlude the tail artery and then released gradually. The pressure at which the tail flow reappeared was recorded as systolic and the pressure at which the tail flow became continuous was recorded as diastolic. Regression analysis of tail-cuff pressures over catheter pressures obtained from healthy mice (n = 16) showed a high degree of association (rsys = 0.95, rdia = 0.94, both at p < 0.001). Bland-Altman analysis showed good agreement between the two methods, with a mean difference of -13 ( ± 12 SD) mmHg and 3 ( ± 10 SD) mmHg in the systolic (58 to 250 mmHg) and diastolic (48 to 178 mmHg) pressure measurements, respectively. Bland-Altman plots of tail-cuff blood pressures of a second group of mice (n = 20) showed good agreement between repeated measurements obtained on the same day, but had higher variability between measurements made on different days.
KW - Doppler mouse tail-flow sensor
KW - Doppler sphygmomanometry
KW - Mouse tail-cuff diastolic pressure
KW - Mouse tail-cuff systolic pressure
KW - Mouse tail-flow velocity
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U2 - 10.1016/S0301-5629(02)00746-9
DO - 10.1016/S0301-5629(02)00746-9
M3 - Article
C2 - 12706189
AN - SCOPUS:0037363190
VL - 29
SP - 379
EP - 385
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
SN - 0301-5629
IS - 3
ER -