Non-virally mediated gene transfer into human central nervous system precursor cells

Elena Cattaneo; Luciano Conti; Angela Gritti; Paola Frolichsthal; Stefano Govoni; Angelo Vescovi

doi:10.1016/S0169-328X(96)00159-3

Non-virally mediated gene transfer into human central nervous system precursor cells

Elena Cattaneo, Luciano Conti, Angela Gritti, Paola Frolichsthal, Stefano Govoni, Angelo Vescovi

Academic Institute

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Lipofectamine-based transfection was used as a method of choice to deliver the bacterial β-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. β-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.

Original language	English (US)
Pages (from-to)	161-166
Number of pages	6
Journal	Molecular Brain Research
Volume	42
Issue number	1
DOIs	https://doi.org/10.1016/S0169-328X(96)00159-3
State	Published - Nov 1996

Keywords

Human CNS precursor cell
Large-T antigen
Lipofectamine
Transfection

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/S0169-328X(96)00159-3

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title = "Non-virally mediated gene transfer into human central nervous system precursor cells",

abstract = "Lipofectamine-based transfection was used as a method of choice to deliver the bacterial β-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4\%. β-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.",

keywords = "Human CNS precursor cell, Large-T antigen, Lipofectamine, Transfection",

author = "Elena Cattaneo and Luciano Conti and Angela Gritti and Paola Frolichsthal and Stefano Govoni and Angelo Vescovi",

note = "Funding Information: This work was supported by the Alzheimer{\textquoteright}s Association/The Hearst Corporation Pilot Research Grant 94-057 to E.C. and partially by funds from The Ministry of Health, Italy. (ICS49.2/RS93.42) to A.V.",

year = "1996",

month = nov,

doi = "10.1016/S0169-328X(96)00159-3",

language = "English (US)",

volume = "42",

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journal = "Molecular Brain Research",

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T1 - Non-virally mediated gene transfer into human central nervous system precursor cells

AU - Cattaneo, Elena

AU - Conti, Luciano

AU - Gritti, Angela

AU - Frolichsthal, Paola

AU - Govoni, Stefano

AU - Vescovi, Angelo

N1 - Funding Information: This work was supported by the Alzheimer’s Association/The Hearst Corporation Pilot Research Grant 94-057 to E.C. and partially by funds from The Ministry of Health, Italy. (ICS49.2/RS93.42) to A.V.

PY - 1996/11

Y1 - 1996/11

N2 - Lipofectamine-based transfection was used as a method of choice to deliver the bacterial β-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. β-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.

AB - Lipofectamine-based transfection was used as a method of choice to deliver the bacterial β-galactosidase gene into human central nervous system (CNS) precursor cells. We achieved a transfection efficiency of 7.4%. β-Galactosidase expressing cells were shown to display both neuronal and glial phenotypes. We also delivered the temperature sensitive allele of SV40 Large-T antigen and obtained a high level of expression of the immortalizing oncoprotein in the cells. Colonies of Large-T antigen immunoreactive cells were indeed visible 10 days after transfection.

KW - Human CNS precursor cell

KW - Large-T antigen

KW - Lipofectamine

KW - Transfection

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JO - Molecular Brain Research

JF - Molecular Brain Research

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ER -

Non-virally mediated gene transfer into human central nervous system precursor cells

Abstract

Keywords

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