NLRP4 negatively regulates type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4

Jun Cui, Yinyin Li, Liang Zhu, Dan Liu, Zhou Songyang, Helen Yicheng Wang, Rongfu Wang

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

Stringent control of the type I interferon signaling pathway is important for maintaining host immune responses and homeostasis, yet the molecular mechanisms responsible for its tight regulation are still poorly understood. Here we report that the pattern-recognition receptor NLRP4 regulated the activation of type I interferon mediated by double-stranded RNA or DNA by targeting the kinase TBK1 for degradation. NLRP4 recruited the E3 ubiquitin ligase DTX4 to TBK1 for Lys48 (K48)-linked polyubiquitination at Lys670, which led to degradation of TBK1. Knockdown of either DTX4 or NLRP4 abrogated K48-linked ubiquitination and degradation of TBK1 and enhanced the phosphorylation of TBK1 and the transcription factor IRF3. Our results identify a previously unrecognized role for NLRP4 in the regulation of type I interferon signaling and provide molecular insight into the mechanisms by which NLRP4-DTX4 targets TBK1 for degradation.

Original languageEnglish (US)
Pages (from-to)387-395
Number of pages9
JournalNature immunology
Volume13
Issue number4
DOIs
StatePublished - Apr 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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