Nitric oxide ejects electrons from the binuclear centre of cytochrome c oxidase by reacting with oxidised copper: A general mechanism for the interaction of copper proteins with nitric oxide?

Chris E. Cooper, Jaume Torres, Martyn A. Sharpe, Michael T. Wilson

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Small increases in NO concentration can inhibit mitochondrial oxygen consumption by reacting at the binuclear haem a3/Cu(B) oxygen reduction site of cytochrome c oxidase. Here we demonstrate that under normal turnover conditions NO reacts initially with the oxidised Cu(B) rather than the haem as We propose that hydration of an initial Cu+/NO+ complex forms nitrite, a proton and Cu(B); the latter ejects an electron from the binuclear centre and results in the observed (100 s-1) reduction of other electron transfer centres in the enzyme (haem a and Cu(A)) These reactions may have implications for the interactions of NO with other topper proteins.

Original languageEnglish (US)
Pages (from-to)281-284
Number of pages4
JournalFEBS Letters
Volume414
Issue number2
DOIs
StatePublished - Sep 8 1997

Keywords

  • Copper enzyme
  • Cytochrome oxidase
  • Electron transfer
  • Heme enzyme
  • Mitochondria
  • Nitric oxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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