Next-generation transgenic mice for optogenetic analysis of neural circuits

Brent Asrican, George J. Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, Bernd Gloss, Riichiro Hira, Carolin Hoffmann, Haruo Kasai, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Shun Qiang Lo, James Mancuso, Masanori Matsuzaki, Ryuichi Nakajima, Li QiuGregory Tan, Yanxia Tang, Jonathan T. Ting, Sachiko Tsuda, Lei Wen, Xuying Zhang, Shengli Zhao

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.

Original languageEnglish (US)
Article number160
JournalFrontiers in Neural Circuits
Volume7
Issue numberNOV
DOIs
StatePublished - Nov 26 2013

Keywords

  • Cerebellum
  • Channelrhodopsin
  • Cortex
  • Hippocampus
  • Optogenetics
  • Photoinhibition
  • Photostimulation
  • Pons

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Cellular and Molecular Neuroscience
  • Sensory Systems
  • Cognitive Neuroscience

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