Next-generation transgenic mice for optogenetic analysis of neural circuits

Brent Asrican; George J. Augustine; Ken Berglund; Susu Chen; Nick Chow; Karl Deisseroth; Guoping Feng; Bernd Gloss; Riichiro Hira; Carolin Hoffmann; Haruo Kasai; Malvika Katarya; Jinsook Kim; John Kudolo; Li Ming Lee; Shun Qiang Lo; James Mancuso; Masanori Matsuzaki; Ryuichi Nakajima; Li Qiu; Gregory Tan; Yanxia Tang; Jonathan T. Ting; Sachiko Tsuda; Lei Wen; Xuying Zhang; Shengli Zhao

doi:10.3389/fncir.2013.00160

Next-generation transgenic mice for optogenetic analysis of neural circuits

Brent Asrican, George J. Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, Bernd Gloss, Riichiro Hira, Carolin Hoffmann, Haruo Kasai, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Shun Qiang Lo, James Mancuso, Masanori Matsuzaki, Ryuichi Nakajima, Li QiuGregory Tan, Yanxia Tang, Jonathan T. Ting, Sachiko Tsuda, Lei Wen, Xuying Zhang, Shengli Zhao

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57 Scopus citations

Abstract

Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.

Original language	English (US)
Article number	160
Journal	Frontiers in Neural Circuits
Volume	7
Issue number	NOV
DOIs	https://doi.org/10.3389/fncir.2013.00160
State	Published - Nov 26 2013

Keywords

Cerebellum
Channelrhodopsin
Cortex
Hippocampus
Optogenetics
Photoinhibition
Photostimulation
Pons

ASJC Scopus subject areas

Neuroscience (miscellaneous)
Cellular and Molecular Neuroscience
Sensory Systems
Cognitive Neuroscience

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10.3389/fncir.2013.00160

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Asrican, B., Augustine, G. J., Berglund, K., Chen, S., Chow, N., Deisseroth, K., Feng, G., Gloss, B., Hira, R., Hoffmann, C., Kasai, H., Katarya, M., Kim, J., Kudolo, J., Lee, L. M., Lo, S. Q., Mancuso, J., Matsuzaki, M., Nakajima, R., ... Zhao, S. (2013). Next-generation transgenic mice for optogenetic analysis of neural circuits. Frontiers in Neural Circuits, 7(NOV), Article 160. https://doi.org/10.3389/fncir.2013.00160

Asrican, B, Augustine, GJ, Berglund, K, Chen, S, Chow, N, Deisseroth, K, Feng, G, Gloss, B, Hira, R, Hoffmann, C, Kasai, H, Katarya, M, Kim, J, Kudolo, J, Lee, LM, Lo, SQ, Mancuso, J, Matsuzaki, M, Nakajima, R, Qiu, L, Tan, G, Tang, Y, Ting, JT, Tsuda, S, Wen, L, Zhang, X & Zhao, S 2013, 'Next-generation transgenic mice for optogenetic analysis of neural circuits', Frontiers in Neural Circuits, vol. 7, no. NOV, 160. https://doi.org/10.3389/fncir.2013.00160

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abstract = "Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both in vitro and in vivo. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.",

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AU - Asrican, Brent

AU - Augustine, George J.

AU - Berglund, Ken

AU - Chen, Susu

AU - Chow, Nick

AU - Deisseroth, Karl

AU - Feng, Guoping

AU - Gloss, Bernd

AU - Hira, Riichiro

AU - Hoffmann, Carolin

AU - Kasai, Haruo

AU - Katarya, Malvika

AU - Kim, Jinsook

AU - Kudolo, John

AU - Lee, Li Ming

AU - Lo, Shun Qiang

AU - Mancuso, James

AU - Matsuzaki, Masanori

AU - Nakajima, Ryuichi

AU - Qiu, Li

AU - Tan, Gregory

AU - Tang, Yanxia

AU - Ting, Jonathan T.

AU - Tsuda, Sachiko

AU - Wen, Lei

AU - Zhang, Xuying

AU - Zhao, Shengli

PY - 2013/11/26

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KW - Cerebellum

KW - Channelrhodopsin

KW - Cortex

KW - Hippocampus

KW - Optogenetics

KW - Photoinhibition

KW - Photostimulation

KW - Pons

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Next-generation transgenic mice for optogenetic analysis of neural circuits

Abstract

Keywords

ASJC Scopus subject areas

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