New polymer of lactic-co-glycolic acid-modified polyethylenimine for nucleic acid delivery

Jian Ming Lü, Zhengdong Liang, Xiaoxiao Wang, Jianhua Gu, Qizhi Yao, Changyi Chen

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Aim: To develop an improved delivery system for nucleic acids. Materials & methods: We designed, synthesized and characterized a new polymer of lactic-co-glycolic acid-modified polyethylenimine (LGA-PEI). Functions of LGA-PEI polymer were determined. Results: The new LGA-PEI polymer spontaneously formed nanoparticles (NPs) with DNA or RNA, and showed higher DNA or RNA loading efficiency, higher or comparable transfection efficacy, and lower cytotoxicity in several cell types including PANC-1, Jurkat and HEK293 cells, when compared with lipofectamine 2000, branched or linear PEI (25 kDa). In nude mouse models, LGA-PEI showed higher delivery efficiency of plasmid DNA or miRNA mimic into pancreatic and ovarian xenograft tumors. LGA-PEI/DNA NPs showed much lower toxicity than control PEI NPs in mouse models. Conclusion: The new LGA-PEI polymer is a safer and more effective system to deliver DNA or RNA than PEI.

Original languageEnglish (US)
Pages (from-to)1971-1991
Number of pages21
JournalNanomedicine
Volume11
Issue number15
DOIs
StatePublished - Aug 2016

Keywords

  • LGA-PEI polymer
  • lactic-co-glycolic acid
  • nanoparticle
  • nucleic acid delivery
  • polyethylenimine
  • xenograft tumors

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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