Neutral DNA-avidin nanoparticles as ultrasensitive reporters in immuno-PCR

Dimple Chavan, Hui Chen, Mary Crum, Binh Vu, Mohammad Safari, Maxwell Smith, Peter Vekilov, Jacinta C. Conrad, Katerina Kourentzi, Richard C. Willson

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed an immuno-PCR based diagnostic platform which couples detection antibodies to self-assembled, ultra-detectable DNA-avidin nanoparticles stabilized with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific "stickiness" and enhances assay sensitivity. We further optimized the detectability of the nanoparticles by incorporating four repeats of a unique synthetic DNA PCR target into each nanoparticle. Using human chorionic gonadotropin hormone (hCG) as a model analyte, this platform was able to quantitate the target hCG protein in femtomolar concentrations using only standard laboratory equipment.

Original languageEnglish (US)
Pages (from-to)4942-4949
Number of pages8
JournalAnalyst
Volume145
Issue number14
DOIs
StatePublished - Jul 21 2020

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Fingerprint Dive into the research topics of 'Neutral DNA-avidin nanoparticles as ultrasensitive reporters in immuno-PCR'. Together they form a unique fingerprint.

Cite this