TY - JOUR
T1 - Neurotrophic effects of hippocampal extracts on medial septal nucleus in vitro
AU - Ojika, K.
AU - Appel, Stanley H.
PY - 1984
Y1 - 1984
N2 - Within peripheral sympathetic and sensory systems, target tissue provides diffusible factors such as nerve growth factor that influence neuronal survival, growth, and differentiation. To determine whether target tissue may exert retrograde effects within the central nervous system, we have used hippocampus as a source of neurotrophic factors and cultured medial septal nucleus explants as a source of neurons that may respond to such factors. Soluble extracts from rat hippocampus enhance cholinergic activity (choline acetyltransferase, choline uptake, acetylcholine synthesis) of the rat medial septal nucleus cultured in serum-free defined medium. The enhancement is dose dependent, relatively specific for hippocampus, and varies with age, reaching a peak in 2- to 3-week-old rat hippocampus. The enhancement activity of the hippocampal extract appears to be mediated by protease-sensitive polypeptide(s) that differ from nerve growth factor in biological and chemical characteristics.
AB - Within peripheral sympathetic and sensory systems, target tissue provides diffusible factors such as nerve growth factor that influence neuronal survival, growth, and differentiation. To determine whether target tissue may exert retrograde effects within the central nervous system, we have used hippocampus as a source of neurotrophic factors and cultured medial septal nucleus explants as a source of neurons that may respond to such factors. Soluble extracts from rat hippocampus enhance cholinergic activity (choline acetyltransferase, choline uptake, acetylcholine synthesis) of the rat medial septal nucleus cultured in serum-free defined medium. The enhancement is dose dependent, relatively specific for hippocampus, and varies with age, reaching a peak in 2- to 3-week-old rat hippocampus. The enhancement activity of the hippocampal extract appears to be mediated by protease-sensitive polypeptide(s) that differ from nerve growth factor in biological and chemical characteristics.
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U2 - 10.1073/pnas.81.8.2567
DO - 10.1073/pnas.81.8.2567
M3 - Article
C2 - 6585816
AN - SCOPUS:0021416449
SN - 0027-8424
VL - 81
SP - 2567
EP - 2571
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 8 I
ER -